Enzymes Recombinant Proteins

Human CNDP1 Recombinant Protein (RPPB1499)

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RPPB1499

Description

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Product Name:Human CNDP1 Recombinant Protein
Product Code:RPPB1499
Size:10µg
Species:Human
Target:CNDP1
Synonyms:CarnosineDipeptidase 1 (Metallopeptidase M20 Family), Glutamate Carboxypeptidase-LikeProtein 2, CNDP Dipeptidase 1, Serum Carnosinase, Carnosinase 1, CPGL2, CN1,Carnosine Dipeptidase 1, EC 3.4.13.20, HsT2308.
Source:Sf9 Insect cells
Physical Appearance:Sterilefiltered colorless solution.
Formulation:CNDP1 protein solution (0.25mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greaterthan 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:SPSPPPALLE KVFQYIDLHQ DEFVQTLKEW VAIESDSVQP VPRFRQELFR MMAVAADTLQ RLGARVASVD MGPQQLPDGQ SLPIPPVILA ELGSDPTKGT VCFYGHLDVQ PADRGDGWLT DPYVLTEVDG KLYGRGATDN KGPVLAWINA VSAFRALEQD LPVNIKFIIE GMEEAGSVAL EELVEKEKDR FFSGVDYIVI SDNLWISQRK PAITYGTRGN SYFMVEVKCR DQDFHSGTFG GILHEPMADL VALLGSLVDS SGHILVPGIY DEVVPLTEEE INTYKAIHLD LEEYRNSSRV EKFLFDTKEE ILMHLWRYPS LSIHGIEGAF DEPGTKTVIP GRVIGKFSIR LVPHMNVSAV EKQVTRHLED VFSKRNSSNK MVVSMTLGLH PWIANIDDTQ YLAAKRAIRT VFGTEPDMIR DGSTIPIAKM FQEIVHKSVV LIPLGAVDDG EHSQNEKINR WNYIEGTKLF AAFFLEMAQL HLEHHHHHH
Biological Activity:Specific activity is >3,000 pmol/min/ug, and as measured by the hydrolysis of carnosine per minute at pH6.8 at 25�C.

CNDP Dipeptidase 1, also known as CNDP1 is a member of the peptidaseM20A family. CNDP1 Mannheim which is the shortest allelic form has been morecommon in the absence of nephropathy in addition to being associated with lower serum carnosinase levels. Furthermore, Carnosine inhibited the increasedproduction of fibronectin as well as collagen type VI in podocytes and theincreased production of TGF-beta in mesangial cells. Diabetic patients with theCNDP1 Mannheim variant are less at risk for nephropathy. In addition, on renalcells carnosine protects against the adverse effects of high glucose levels.

CNDP1 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 489 amino acids (27-507 a.a.) and having a molecular mass of 54.9kDa (Migrates at 50-70kDa on SDS-PAGE under reducing conditions).CNDP1 is expressed with a 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:CNDP1: a member of the M20 metalloprotease family. The encoded protein is specifically expressed in the brain, is a homodimeric dipeptidase which was identified as human carnosinase. This gene contains trinucleotide (CTG) repeat length polymorphism in the coding region. [provided by RefSeq, Jul 2008]
UniProt Protein Details:

Protein type:Amino Acid Metabolism - histidine; Protease; Secreted, signal peptide; Secreted; Other Amino Acids Metabolism - beta-alanine; EC 3.4.13.20

Chromosomal Location of Human Ortholog: 18q22.3

Cellular Component: extracellular region

Molecular Function:dipeptidase activity; metallopeptidase activity; carboxypeptidase activity; metal ion binding

Biological Process: proteolysis

UniProt Code:Q96KN2
NCBI GenInfo Identifier:21071039
NCBI Gene ID:84735
NCBI Accession:NP_116038.4
UniProt Secondary Accession:Q96KN2,Q14D40, Q17S05, Q2TBG0, Q6UWK2, Q9BT98,
UniProt Related Accession:Q96KN2
Molecular Weight:56,706 Da
NCBI Full Name:beta-Ala-His dipeptidase
NCBI Synonym Full Names:carnosine dipeptidase 1 (metallopeptidase M20 family)
NCBI Official Symbol:CNDP1��
NCBI Official Synonym Symbols:CN1; CPGL2; HsT2308��
NCBI Protein Information:beta-Ala-His dipeptidase
UniProt Protein Name:Beta-Ala-His dipeptidase
UniProt Synonym Protein Names:CNDP dipeptidase 1; Carnosine dipeptidase 1; Glutamate carboxypeptidase-like protein 2; Serum carnosinase
Protein Family:Beta-Ala-His dipeptidase
UniProt Gene Name:CNDP1��
UniProt Entry Name:CNDP1_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q96KN2
Research Area:
Enzymes
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