Enzymes Recombinant Proteins

Human CMBL Recombinant Protein (RPPB1497)

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RPPB1497

Description

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Product Name:Human CMBL Recombinant Protein
Product Code:RPPB1497
Size:20µg
Species:Human
Target:CMBL
Synonyms:Carboxymethylenebutenolidase homolog, CMBL, JS-1.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:The CMBL solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 10% glycerol and 1mM EDTA.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMANEAY PCPCDIGHRL EYGGLGREVQ VEHIKAYVTK SPVDAGKAVI VIQDIFGWQL PNTRYIADMI SGNGYTTIVP DFFVGQEPWD PSGDWSIFPE WLKTRNAQKI DREISAILKY LKQQCHAQKI GIVGFCWGGT AVHHLMMKYS EFRAGVSVYG IVKDSEDIYN LKNPTLFIFA ENDVVIPLKD VSLLTQKLKE HCKVEYQIKT FSGQTHGFVH RKREDCSPAD KPYIDEARRN LIEWLNKYM

Carboxymethylenebutenolidase homolog (CMBL) is a cysteine hydrolase of the dienelactone hydrolase family which is highly expressed in the liver cytosol. CMBL is the human homolog of Pseudomonas dienelactone hydrolase, which is a protein that participates in the bacterial halocatechol degradation pathway. CMBL which preferentially cleaves cyclic esters activates medoxomil-ester prodrugs in which the medoxomil moiety is coupled with an oxygen atom. CMBL is inhibited by PCMB (p-chloromercuribenzoate) and is encoded by a gene which maps to human chromosome 5p15.2. CMBL can also activate beta-lactam antibiotics faropenem medoxomil and lenampicillin. CMBL is widely expressed, with the highest levels in the liver, followed by the kidney, small intestine and the colon.

CMBL Human Recombinant produced in E. coli is a single polypeptide chain containing 269 amino acids (1-245) and having a molecular mass of 30.6kDa.CMBL is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:CMBL: Cysteine hydrolase. Can convert the prodrug olmesartan medoxomil into its pharmacologically active metabolite olmerstatan, an angiotensin receptor blocker, in liver and intestine. May also activate beta-lactam antibiotics faropenem medoxomil and lenampicillin. Belongs to the dienelactone hydrolase family.Protein type: Hydrolase; EC 3.1.-.-Chromosomal Location of Human Ortholog: 5p15.2Cellular Component: cytosolMolecular Function: hydrolase activityBiological Process: xenobiotic metabolic process
UniProt Protein Details:
NCBI Summary:CMBL (EC 3.1.1.45) is a cysteine hydrolase of the dienelactone hydrolase family that is highly expressed in liver cytosol. CMBL preferentially cleaves cyclic esters, and it activates medoxomil-ester prodrugs in which the medoxomil moiety is linked to an oxygen atom (Ishizuka et al., 2010 [PubMed 20177059]).[supplied by OMIM, Apr 2010]
UniProt Code:Q96DG6
NCBI GenInfo Identifier:20270371
NCBI Gene ID:134147
NCBI Accession:NP_620164.1
UniProt Secondary Accession:Q96DG6,Q8TED6, D3DTC7
UniProt Related Accession:Q96DG6
Molecular Weight:28 kDa
NCBI Full Name:carboxymethylenebutenolidase homolog
NCBI Synonym Full Names:carboxymethylenebutenolidase homolog (Pseudomonas)
NCBI Official Symbol:CMBL��
NCBI Official Synonym Symbols:JS-1��
NCBI Protein Information:carboxymethylenebutenolidase homolog
UniProt Protein Name:Carboxymethylenebutenolidase homolog
UniProt Synonym Protein Names:
Protein Family:Carboxymethylenebutenolidase
UniProt Gene Name:CMBL��
UniProt Entry Name:CMBL_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q96DG6
Research Area:
Enzymes
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