Human CLTB Recombinant Protein (RPPB3210)

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SKU:
RPPB3210
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P09497

Description

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Product Name:Human CLTB Recombinant Protein
Product Code:RPPB3210
Size:20µg
Species:Human
Target:CLTB
Synonyms:Clathrin light chain B, Lcb, CLTB.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:CLTB protein solution (1mg/ml) containing 20mM Tris-HCl buffer, pH8.0, 10% glycerol, 0.2mM PMSF and 100mM NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMADDFGF FSSSESGAPE AAEEDPAAAF LAQQESEIAG IENDEGFGAP AGSHAAPAQP GPTSGAGSED MGTTVNGDVF QEANGPADGY AAIAQADRLT QEPESIRKWR EEQRKRLQEL DAASKVTEQE WREKAKKDLE EWNQRQSEQV EKNKINNRAS EEAFVKESKE ETPGTEWEKV AQLCDFNPKS SKQCKDVSRL RSVLMSLKQT PLSR

Clathrin light chain B (CLTB) is a member of the clathrin light chain family. Clathrin is a protein that has a key role in the formation of coated vesicles. CLTB functions as the key structural component of the lattice-type cytoplasmic face of coated pits and vesicles which capture certain macromolecules during receptor-mediated endocytosis. CLTB forms a triskelion form comprised of 3 clathrin heavy chains and 3 light chains. When the triskelia interact they form a polyhedral pattern which encircles the vesicle. CME (Clathrin-mediated endocytosis) controls numerous cellular physiological processes such as the internalization of growth factors and receptors, entry of pathogens, and synaptic transmission.

CLTB Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 234 amino acids (1-211 a.a) and having a molecular mass of 25.6kDa (Molecular size on SDS-PAGE will appear higher).CLTB is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:CLTB: Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Clathrin coats are formed from molecules containing 3 heavy chains and 3 light chains. Interacts (via N-terminus) with HIP1. Interacts with HIP1R. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Vesicle

Chromosomal Location of Human Ortholog: 5q35

Cellular Component: clathrin coat; clathrin coat of coated pit; clathrin coat of trans-Golgi network vesicle; clathrin vesicle coat; cytoplasm; intracellular membrane-bound organelle; plasma membrane; trans-Golgi network

Molecular Function:clathrin heavy chain binding; peptide binding; protein binding; structural molecule activity

Biological Process: intracellular protein transport

NCBI Summary:Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]
UniProt Code:P09497
NCBI GenInfo Identifier:4502901
NCBI Gene ID:1212
NCBI Accession:NP_001825.1
UniProt Secondary Accession:P09497,Q53Y37, Q6FHW1,
UniProt Related Accession:P09497
Molecular Weight:23,181 Da
NCBI Full Name:clathrin light chain B isoform a
NCBI Synonym Full Names:clathrin light chain B
NCBI Official Symbol:CLTB��
NCBI Official Synonym Symbols:LCB��
NCBI Protein Information:clathrin light chain B
UniProt Protein Name:Clathrin light chain B
Protein Family:Clathrin
UniProt Gene Name:CLTB��
UniProt Entry Name:CLCB_HUMAN
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