Human CLPS Recombinant Protein (RPPB3208)

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RPPB3208

Description

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Product Name:Human CLPS Recombinant Protein
Product Code:RPPB3208
Size:20µg
Species:Human
Target:CLPS
Synonyms:Colipase Pancreatic, Pancreatic Colipase Preproprotein, Colipase, CLPS.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The CLPS solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 30% glycerol, 1mM DTT and 0.1M NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSAPGPRGI IINLENGELC MNSAQCKSNC CQHSSALGLA RCTSMASENS ECSVKTLYGI YYKCPCERGL TCEGDKTIVG SITNTNFGIC HDAGRSKQ

Colipase (CLPS) is a protein co-enzyme essential for optimal enzyme activity of pancreatic lipase. CLPS is secreted by the pancreas as an inactive form, procolipase, which is then activated in the intestinal lumen by trypsin. CLPS prevents the inhibitory effect of bile salts on the lipase-catalyzed intraduodenal hydrolysis of dietary long-chain triglycerides. CLPS allows lipase to anchor noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts. CLPS binds to the C-terminal, non-catalytic domain of lipase, thus stabilizing an active conformation and significantly increasing the whole hydrophobic binding site.

CLPS Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 118 amino acids (18-112) and having a molecular mass of 12.5 kDa.CLPS is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:CLPS: Colipase is a cofactor of pancreatic lipase. It allows the lipase to anchor itself to the lipid-water interface. Without colipase the enzyme is washed off by bile salts, which have an inhibitory effect on the lipase. Belongs to the colipase family.
UniProt Protein Details:

Protein type:Activator; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6p21.31

Cellular Component: extracellular region

Molecular Function:enzyme activator activity

Biological Process: positive regulation of catalytic activity; phototransduction, visible light; lipid metabolic process; retinoid metabolic process; lipid catabolic process; lipid digestion

NCBI Summary:The protein encoded by this gene is a cofactor needed by pancreatic lipase for efficient dietary lipid hydrolysis. It binds to the C-terminal, non-catalytic domain of lipase, thereby stabilizing an active conformation and considerably increasing the overall hydrophobic binding site. The gene product allows lipase to anchor noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts. This cofactor is only expressed in pancreatic acinar cells, suggesting regulation of expression by tissue-specific elements. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
UniProt Code:P04118
NCBI GenInfo Identifier:116900
NCBI Gene ID:1208
NCBI Accession:P04118.2
UniProt Secondary Accession:P04118,Q5T9G7, Q5U809,
UniProt Related Accession:P04118
Molecular Weight:112
NCBI Full Name:Colipase
NCBI Synonym Full Names:colipase, pancreatic
NCBI Official Symbol:CLPS��
NCBI Protein Information:colipase; pancreatic colipase preproprotein
UniProt Protein Name:Colipase
Protein Family:Colipase
UniProt Gene Name:CLPS��
UniProt Entry Name:COL_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P04118
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