Human Cleaved CASP8 (Cleaved Caspase-8) ELISA Kit (HUFI04741)
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- SKU:
- HUFI04741
Description
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Human Cleaved CASP8 (Cleaved Caspase-8) ELISA Kit
Cleaved CASP8 (Cleaved Caspase-8) is a biomarker for early detection of ovarian cancer. Diseases associated with CASP8 include Caspase 8 Deficiency and Hepatocellular Carcinoma. Among Cleaved CASP8 gene related pathways are Activated TLR4 signalling and Nanoparticle triggered regulated necrosis. The Assay Genie Human Cleaved CASP8 (Cleaved Caspase-8) ELISA is a highly sensitive assay for the quantitative measurement of Cleaved CASP8 (Cleaved Caspase-8) in serum, blood, plasma, cell culture supernatant and tissue samples.
| Product Name: | Human Cleaved CASP8 (Cleaved Caspase-8) ELISA Kit |
| Product Code: | HUFI04741 |
| Size: | 96 Assays |
| Alias: | Cleaved CASP8 ELISA Kit |
| Detection method: | Sandwich ELISA, Double Antibody |
| Application: | This immunoassay kit allows for the in vitro quantitative determination of Human Cleaved CASP8 (Cleaved Caspase-8) concentrations in serum plasma and other biological fluids. |
| Sensitivity: | < 18.75pg/ml |
| Range: | 31.25-2000pg/ml |
| Storage: | 4°C for 6 months |
| Note: | For Research Use Only |
| Recovery: | Matrices listed below were spiked with certain level of Human Cleaved CASP8 (Cleaved Caspase-8) and the recovery rates were calculated by comparing the measured value to the expected amount of Human Cleaved CASP8 (Cleaved Caspase-8) in samples. | ||||||||||||||||
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| Linearity: | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human Cleaved CASP8 (Cleaved Caspase-8) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. | ||||||||||||||||
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| CV(%): | Intra-Assay: CV<8% Inter-Assay: CV<10% |
| Component | Quantity | Storage |
| ELISA Microplate (Dismountable) | 8×12 strips | 4°C for 6 months |
| Lyophilized Standard | 2 | 4°C/-20°C |
| Sample/Standard Dilution Buffer | 20ml | 4°C |
| Biotin-labeled Antibody(Concentrated) | 120ul | 4°C (Protect from light) |
| Antibody Dilution Buffer | 10ml | 4°C |
| HRP-Streptavidin Conjugate(SABC) | 120ul | 4°C (Protect from light) |
| SABC Dilution Buffer | 10ml | 4°C |
| TMB Substrate | 10ml | 4°C (Protect from light) |
| Stop Solution | 10ml | 4°C |
| Wash Buffer(25X) | 30ml | 4°C |
| Plate Sealer | 5 | - |
Other materials and equipment required:
- Microplate reader with 450 nm wavelength filter
- Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
- Incubator
- Deionized or distilled water
- Absorbent paper
- Buffer resevoir
| UniProt Protein Function: | CASP8: Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death- inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp--AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex. Heterotetramer that consists of two anti-parall Fill out our quote form below and a dedicated member of staff will get back to you within one working day! Additional Information
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 Human Cleaved CASP9 (Cleaved Caspase-9) ELISA Kit (HUFI04740)
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