Enzymes Recombinant Proteins

Human CKMT2 Recombinant Protein (RPPB2486)

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RPPB2486

Description

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Product Name:Human CKMT2 Recombinant Protein
Product Code:RPPB2486
Size:20µg
Species:Human
Target:CKMT2
Synonyms:Creatine kinase mitochondrial 2 (sarcomeric), Basic-type mitochondrial creatine kinase, Sarcomeric mitochondrial creatine kinase, creatine kinase S-type, mitochondrial, SMTCK, Mib-CK, EC 2.7.3.2.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The CKMT2 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 1mM DTT and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMEVREQ PRLFPPSADY PDLRKHNNCM AECLTPAIYA KLRNKVTPNG YTLDQCIQTG VDNPGHPFIK TVGMVAGDEE SYEVFADLFD PVIKLRHNGY DPRVMKHTTD LDASKITQGQ FDEHYVLSSR VRTGRSIRGL SLPPACTRAE RREVENVAIT ALEGLKGDLA GRYYKLSEMT EQDQQRLIDD HFLFDKPVSP LLTCAGMARD WPDARGIWHN YDKTFLIWIN EEDHTRVISM EKGGNMKRVF ERFCRGLKEV ERLIQERGWE FMWNERLGYI LTCPSNLGTG LRAGVHVRIP KLSKDPRFSK ILENLRLQKR GTGGVDTAAV ADVYDISNID RIGRSEVELV QIVIDGVNYL VDCEKKLERG QDIKVPPPLP QFGKK

Creatine Kinase, Mitochondrial 2 (CKMT2) is a member of the ATP:guanido phosphotransferase family. CKMT2 is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. CKMT2 reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes have a principal role in energy transduction in tissues with large, variable energy demands, such as skeletal muscle, heart, brain and spermatozoa. Mitochondrial creatine kinase occurs in 2 different oligomeric forms: dimers and octamers, contrary to the exclusively dimeric cytosolic creatine kinase isoenzymes. The CKMT2 gene contains sequences homologous to a number of motifs which are shared among some nuclear genes encoding mitochondrial proteins and therefore may be crucial for the coordinated activation of these genes during mitochondrial biogenesis.

CKMT2 Human Recombinant produced in E. coli is a single polypeptide chain containing 405 amino acids (40-419) and having a molecular mass of 46.1 kDa.CKMT2 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:CKMT2: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family.
UniProt Protein Details:

Protein type:Mitochondrial; Amino Acid Metabolism - arginine and proline; Kinase, other; EC 2.7.3.2

Chromosomal Location of Human Ortholog: 5q13.3

Cellular Component: mitochondrial inner membrane; mitochondrion

Molecular Function:creatine kinase activity

Biological Process: creatine metabolic process; muscle contraction

NCBI Summary:Mitochondrial creatine kinase (MtCK) is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Sarcomeric mitochondrial creatine kinase has 80% homology with the coding exons of ubiquitous mitochondrial creatine kinase. This gene contains sequences homologous to several motifs that are shared among some nuclear genes encoding mitochondrial proteins and thus may be essential for the coordinated activation of these genes during mitochondrial biogenesis. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P17540
NCBI GenInfo Identifier:116242603
NCBI Gene ID:1160
NCBI Accession:P17540.2
UniProt Secondary Accession:P17540,Q6ICS8, Q8N1E1,
UniProt Related Accession:P17540
Molecular Weight:47,504 Da
NCBI Full Name:Creatine kinase S-type, mitochondrial
NCBI Synonym Full Names:creatine kinase, mitochondrial 2
NCBI Official Symbol:CKMT2��
NCBI Official Synonym Symbols:SMTCK��
NCBI Protein Information:creatine kinase S-type, mitochondrial
UniProt Protein Name:Creatine kinase S-type, mitochondrial
UniProt Synonym Protein Names:Basic-type mitochondrial creatine kinase; Mib-CK; Sarcomeric mitochondrial creatine kinase; S-MtCK
Protein Family:Creatine kinase
UniProt Gene Name:CKMT2��
UniProt Entry Name:KCRS_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P17540
Research Area:
Enzymes
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