Enzymes Recombinant Proteins

Human CKMT1A Recombinant Protein (RPPB2485)

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RPPB2485

Description

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Product Name:Human CKMT1A Recombinant Protein
Product Code:RPPB2485
Size:20µg
Species:Human
Target:CKMT1A
Synonyms:Creatine kinase mitochondrial 1A, creatine kinase mitochondrial 1 (ubiquitous), creatine kinase U-type mitochondrial, Acidic-type mitochondrial creatine kinase, Ubiquitous mitochondrial creatine kinase, CKMT1, U-MtCK, mia-CK, EC 2.7.3, EC 2.7.3.2.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The CKMT1A solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMASERR RLYPPSAEYP DLRKHNNCMA SHLTPAVYAR LCDKTTPTGW TLDQCIQTGV DNPGHPFIKT VGMVAGDEET YEVFADLFDP VIQERHNGYD PRTMKHTTDL DASKIRSGYF DERYVLSSRV RTGRSIRGLS LPPACTRAER REVERVVVDA LSGLKGDLAG RYYRLSEMTE AEQQQLIDDH FLFDKPVSPL LTAAGMARDW PDARGIWHNN EKSFLIWVNE EDHTRVISME KGGNMKRVFE RFCRGLKEVE RLIQERGWEF MWNERLGYIL TCPSNLGTGL RAGVHIKLPL LSKDSRFPKI LENLRLQKRG TGGVDTAATG GVFDISNLDR LGKSEVELVQ LVIDGVNYLI DCERRLERGQ DIRIPTPVIH TKH

CKMT1A is in charge of the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. CKMT1A is a member of the creatine kinase isoenzyme family and exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase arises in two different oligomeric forms: dimers and octamers, unlike the exclusively dimeric cytosolic creatine kinase isoenzymes. Numerous malignant cancers with poor prognosis have displayed overexpression of ubiquitous mitochondrial creatine kinase which is linked to high energy turnover and inability to remove cancer cells through apoptosis.

CKMT1A Human Recombinant produced in E. coli is a single polypeptide chain containing 403 amino acids (40-417) and having a molecular mass of 45.0 kDa.CKMT1A is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
NCBI Summary:Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins. [provided by RefSeq, Jul 2008]
UniProt Code:P12532
NCBI GenInfo Identifier:125315
NCBI Gene ID:548596
NCBI Accession:P12532.1
UniProt Secondary Accession:P12532,Q0VAM3, Q32NF6, Q53FC4, B4DIT8, B7ZA09,
UniProt Related Accession:P12532
Molecular Weight:50,421 Da
NCBI Full Name:Creatine kinase U-type, mitochondrial
NCBI Synonym Full Names:creatine kinase, mitochondrial 1A
NCBI Official Symbol:CKMT1A��
NCBI Official Synonym Symbols:CKMT1; U-MtCK; mia-CK��
NCBI Protein Information:creatine kinase U-type, mitochondrial
UniProt Protein Name:Creatine kinase U-type, mitochondrial
UniProt Synonym Protein Names:Acidic-type mitochondrial creatine kinase; Mia-CK; Ubiquitous mitochondrial creatine kinase; U-MtCK
UniProt Gene Name:CKMT1A��
UniProt Entry Name:KCRU_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P12532
Research Area:
Enzymes
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