Enzymes Recombinant Proteins

Human CKB Recombinant Protein (RPPB2479)

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SKU:
RPPB2479
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P12277
Research Area:
Enzymes

Description

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Product Name:Human CKB Recombinant Protein
Product Code:RPPB2479
Size:50µg
Species:Human
Target:CKB
Synonyms:Creatine kinase B-type, EC 2.7.3.2, Creatine kinase B chain, B-CK, CKB, CKBB, CKBBI.
Source:Pichia Pastoris
Physical Appearance:Sterile Filtered colourless liquid formulation.
Formulation:CKBB Human contains 10Mm Bis-Tris-HCl pH-6.0, 50% glycerol, 0.5mM EDTA and 0.5mM DTT.
Stability:CKBB should be stored below -18°C. Please prevent freeze-thaw cycles.
Purity:Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Biological Activity:The biological activity measured by the enzymatic activity of Creatine phosphokinase procedure No.45-UV, 1IU-1 �mole creatine phosphate was 854 IU/mg at 37 degrees celsius corresponding to a Specific Activity of 1,171ng/ml.

Creatine Kinase BB is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized.

CKBB Human Recombinant produced in Pichia Pastoris is a dimeric glycosylated full length polypeptide chain comprised of 2 identical B subunits and having an identical amino acid sequence compared to the native enzyme, purified under non-denaturing conditions and having a Mw of 47kDaThe CKBB is purified by proprietary chromatographic techniques.

UniProt Protein Function:Function: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
UniProt Protein Details:

Catalytic activity: ATP + creatine = ADP + phosphocreatine.

Subunit structure: Dimer of identical or non-identical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.

Subcellular location: Cytoplasm.

Sequence similarities: Belongs to the ATP:guanido phosphotransferase family.Contains 1 phosphagen kinase C-terminal domain.Contains 1 phosphagen kinase N-terminal domain.

NCBI Summary:The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. [provided by RefSeq]
UniProt Code:P12277
NCBI GenInfo Identifier:125294
NCBI Gene ID:1152
NCBI Accession:P12277.1
UniProt Secondary Accession:P12277,Q2LE07, Q6FG40, Q9UC66, A8K236, B2R5R4,
UniProt Related Accession:P12277
Molecular Weight:42,644 Da
NCBI Full Name:Creatine kinase B-type
NCBI Synonym Full Names:creatine kinase, brain
NCBI Official Symbol:CKB��
NCBI Official Synonym Symbols:B-CK; CKBB��
NCBI Protein Information:creatine kinase B-type; creatine kinase-B; creatine kinase B chain
UniProt Protein Name:Creatine kinase B-type
UniProt Synonym Protein Names:B-CK; Creatine kinase B chain
Protein Family:Creatine kinase
UniProt Gene Name:CKB��
UniProt Entry Name:KCRB_HUMAN
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