Human CHL Recombinant Protein (RPES1460)
- SKU:
- RPES1460
- Product Type:
- Recombinant Protein
- Species:
- Human
Description
system_update_altDatasheet
Human CHL Recombinant Protein
Neural cell adhesion molecule L1-like protein, also known as close homolog of L1 (CHL1) is the prototypic member of the CTF / NF-1 family of transcription factors that serve as a novel calcium signaling pathway-responsive transcription factor and is considered as a member of the largest ctf complementation group, consisting of 30 of 126 ctf mutants isolated. CHL1 is a cell adhesion molecule highly related to L1. It contains structure plan of six extracellular C2-type immunoglobulin (Ig) domains followed by five fibronectin type? domains linked by a single membrane-spanning region to a short cytoplasmic domain. The extracellular portion of CHL1 is higyly glycosylated and involved them in hemophilic disease.
| Product Name: | Human CHL Recombinant Protein (RPES1460) |
| Product Code: | RPES1460 |
| Size: | 20µg |
| Species: | Human |
| Expressed Host: | HEK293 Cells |
| Synonyms: | CALL,L1CAM2,LICAM2 |
| Accession: | AAI04919.1 |
| Sequence: | Met 1-Gln 1080 |
| Fusion tag: | C-His |
| Activity: | Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. When 5 x 10E4 cells/well are added to CHL1 coated plates (0.8 µg/ml and 100 ?l/well), approximately 40%-60% will adhere specifically after 60 minutes at 37?. |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | A DNA sequence encoding the extracellular domain of human CHL1 (AAI04919.1) (Met 1-Gln 1080) was fused with a polyhistidine tag at the C-terminus. |
| Purity: | > 95 % as determined by reducing SDS-PAGE. |
| Mol Mass: | 120 kDa |
| AP Mol Mass: | 160-180 kDa |
| Formulation: | Lyophilized from sterile PBS, pH 7.4 |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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