Description
| Product Name: | Human CHI3L1 (22-383) Recombinant Protein |
| Product Code: | RPPB1491 |
| Size: | 5µg |
| Species: | Human |
| Target: | CHI3L1 (22-383) |
| Synonyms: | Chitinase 3 Like 1, Chitinase 3-Like 1 (CartilageGlycoprotein-39), Cartilage Glycoprotein 39, 39 KDa Synovial Protein, HCGP-39,CGP-39, YKL-40, GP-39 , Chitinase-3-Like Protein 1, Cartilage Glycoprotein-39,HC-Gp39, HCGP-3P, YYL-40, ASRT7, YKL40. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | CHI3L1 protein solution (0.25mg/ml) contains PhosphateBuffered Saline (pH 7.4), 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).�Avoid multiple freeze-thaw cycles. |
| Purity: | Greaterthan 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | YKLVCYYTSW SQYREGDGSC FPDALDRFLC THIIYSFANI SNDHIDTWEWNDVTLYGMLN TLKNRNPNLK TLLSVGGWNF GSQRFSKIAS NTQSRRTFIK SVPPFLRTHG FDGLDLAWLYPGRGDKQHFT TLIKEMKAEF IKEAQPGKKQ LLLSAALSAG KVTIDSSYDI AKISQHLDFI SIMTYDFHGAWRGTTGHHSP LFRGQEDASP DRFSNTDYAV GYMLRLGAPA SKLVMGIPTF GRSFTLASSE TGVGAPISGPGIPGRFTKEA GTLAYYEICD FLRGATVHRI LGQQVPYATK GNQWVGYDDQ ESVKSKVQYL KDRQLAGAMVWALDLDDFQG SFCGQDLRFP LTNAIKDALA ATLEHHHHHH |
Chitinase 3-Like 1 (CHI3L1) catalyze the hydrolysis of chitin that is an abundant glycopolymer present in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases comprises 8 human family members. CHI3L1 belongs to the glycosyl hydrolase 18 family. CHI3L1 lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. CHI3L1 takes part in the process of inflammation and tissue remodeling.
CHI3L1 produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 370 amino acids (22-383 a.a.) andhaving a molecular mass of 41.4kDa (Migrates at 40-57kDa on SDS-PAGE underreducing conditions).CHI3L1 is expressed with an 8 amino acid His tag atC-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CHI3L1: Carbohydrate-binding lectin with a preference for chitin. May play a role in defense against pathogens, or in tissue remodeling. May play an important role in the capacity of cells to respond to and cope with changes in their environment. A genetic variation in CHI3L1 is associated with susceptibility to asthma-related traits type 7 (ASRT7). Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing and dyspnea, bronchial hyperresponsiveness (BHR) as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis. Belongs to the glycosyl hydrolase 18 family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted; Cell adhesion Chromosomal Location of Human Ortholog: 1q32.1 Cellular Component: extracellular space; proteinaceous extracellular matrix; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm Molecular Function:extracellular matrix structural constituent; chitin binding; chitinase activity; carbohydrate binding Biological Process: positive regulation of protein kinase B signaling cascade; positive regulation of angiogenesis; apoptosis; response to mechanical stimulus; cartilage development; carbohydrate metabolic process; activation of NF-kappaB-inducing kinase; immune response; chitin catabolic process; inflammatory response; lung development Disease: Schizophrenia; Asthma-related Traits, Susceptibility To, 7 |
| NCBI Summary: | Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. [provided by RefSeq, Sep 2009] |
| UniProt Code: | P36222 |
| NCBI GenInfo Identifier: | 84028186 |
| NCBI Gene ID: | 1116 |
| NCBI Accession: | P36222.2 |
| UniProt Secondary Accession: | P36222,P30923, Q8IVA4, Q96HI7, B2R7B0, |
| UniProt Related Accession: | P36222 |
| Molecular Weight: | 42,625 Da |
| NCBI Full Name: | Chitinase-3-like protein 1 |
| NCBI Synonym Full Names: | chitinase 3-like 1 (cartilage glycoprotein-39) |
| NCBI Official Symbol: | CHI3L1�� |
| NCBI Official Synonym Symbols: | GP39; ASRT7; GP-39; YKL40; CGP-39; YKL-40; YYL-40; HC-gp39; HCGP-3P; hCGP-39�� |
| NCBI Protein Information: | chitinase-3-like protein 1; 39 kDa synovial protein; cartilage glycoprotein 39 |
| UniProt Protein Name: | Chitinase-3-like protein 1 |
| UniProt Synonym Protein Names: | 39 kDa synovial protein; Cartilage glycoprotein 39; CGP-39; GP-39; hCGP-39; YKL-40 |
| Protein Family: | Chitinase-3-like protein |
| UniProt Gene Name: | CHI3L1�� |
| UniProt Entry Name: | CH3L1_HUMAN |
