Human Chemokine Array (38 targets) (SARB0030)

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SKU:
SARB0030

Description

system_update_altDatasheet
Product Sku:SARB0030
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:38
Gene Symbols:CCL1, CCL11, CCL13, CCL15, CCL16, CCL17, CCL18, CCL19, CCL2, CCL20|CCL22, CCL23, CCL24, CCL25, CCL26, CCL27, CCL28, CCL3, CCL4, CCL5, CCL7, CCL8, CX3CL1, CXCL1, CXCL10, CXCL11, CXCL12, CXCL13, CXCL16, CXCL2, CXCL3, CXCL5, CXCL6, CXCL8, CXCL9, PPBP, XCL1
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
BLC (CXCL13)
  • Gene Symbol: CXCL13
  • Gene ID: 10563
  • Accession #: O43927
CCL28 (MEC)
  • Gene Symbol: CCL28
  • Gene ID: 56477
  • Accession #: Q9NRJ3
Ck beta 8-1 (CCL23)
  • Gene Symbol: CCL23
  • Gene ID: 6368
  • Accession #: P55773
CTACK (CCL27)
  • Gene Symbol: CCL27
  • Gene ID: 10850
  • Accession #: Q9Y4X3
CXCL16
  • Gene Symbol: CXCL16
  • Gene ID: 58191
  • Accession #: Q9H2A7
ENA-78 (CXCL5)
  • Gene Symbol: CXCL5
  • Gene ID: 6374
  • Accession #: P42830
Eotaxin-1 (CCL11)
  • Gene Symbol: CCL11
  • Gene ID: 6356
  • Accession #: P51671
Eotaxin-2 (MPIF-2/CCL24)
  • Gene Symbol: Ccl24
  • Gene ID: 6369
  • Accession #: O00175
Eotaxin-3 (CCL26)
  • Gene Symbol: CCL26
  • Gene ID: 10344
  • Accession #: Q9Y258
Fractalkine (CX3CL1)
  • Gene Symbol: CX3CL1
  • Gene ID: 6376
  • Accession #: P78423
GCP-2 (CXCL6)
  • Gene Symbol: CXCL6
  • Gene ID: 6372
  • Accession #: P80162
GRO alpha/beta/gamma
  • Gene Symbol: CXCL1/CXCL2/CXCL3
  • Gene ID: 2919/2920/2921
  • Accession #: P09341/P19875/P19876
GRO alpha (CXCL1)
  • Gene Symbol: CXCL1
  • Gene ID: 2919
  • Accession #: P09341
HCC-4 (CCL16)
  • Gene Symbol: CCL16
  • Gene ID: 6360
  • Accession #: O15467
I-309 (TCA-3/CCL1)
  • Gene Symbol: CCL1
  • Gene ID: 6346
  • Accession #: P22362
I-TAC (CXCL11)
  • Gene Symbol: CXCL11
  • Gene ID: 6373
  • Accession #: O14625
IL-8 (CXCL8)
  • Gene Symbol: IL8
  • Gene ID: 3576
  • Accession #: P10145
IP-10 (CXCL10)
  • Gene Symbol: CXCL10
  • Gene ID: 3627
  • Accession #: P02778
Lymphotactin (XCL1)
  • Gene Symbol: XCL1
  • Gene ID: 6375
  • Accession #: P47992
MCP-1 (CCL2)
  • Gene Symbol: CCL2
  • Gene ID: 6347
  • Accession #: P13500
MCP-2 (CCL8)
  • Gene Symbol: CCL8
  • Gene ID: 6355
  • Accession #: P80075
MCP-3 (MARC/CCL7)
  • Gene Symbol: CCL7
  • Gene ID: 6354
  • Accession #: P80098
MCP-4 (CCL13)
  • Gene Symbol: CCL13
  • Gene ID: 6357
  • Accession #: Q99616
MDC (CCL22)
  • Gene Symbol: CCL22
  • Gene ID: 6367
  • Accession #: O00626
MIG (CXCL9)
  • Gene Symbol: CXCL9
  • Gene ID: 4283
  • Accession #: Q07325
MIP-1 alpha (CCL3)
  • Gene Symbol: CCL3
  • Gene ID: 6348
  • Accession #: P10147
MIP-1 beta (CCL4)
  • Gene Symbol: CCL4
  • Gene ID: 6351
  • Accession #: P13236
MIP-1 delta (CCL15)
  • Gene Symbol: CCL15
  • Gene ID: 6359
  • Accession #: Q16663
MIP-3 alpha (CCL20)
  • Gene Symbol: CCL20
  • Gene ID: 6364
  • Accession #: P78556
MIP-3 beta (CCL19)
  • Gene Symbol: CCL19
  • Gene ID: 6363
  • Accession #: Q99731
MPIF-1 (CCL23)
  • Gene Symbol: CCL23
  • Gene ID: 6368
  • Accession #: P55773
NAP-2 (PPBP/CXCL7)
  • Gene Symbol: Ppbp
  • Gene ID: 5473
  • Accession #: P02775
PARC (CCL18)
  • Gene Symbol: CCL18
  • Gene ID: 6362
  • Accession #: P55774
RANTES (CCL5)
  • Gene Symbol: CCL5
  • Gene ID: 6352
  • Accession #: P13501
SDF-1 alpha (CXCL12 alpha)
  • Gene Symbol: CXCL12
  • Gene ID: 6387
  • Accession #: P48061
SDF-1 beta (CXCL12 beta)
  • Gene Symbol: CXCL12
  • Gene ID: 6387
  • Accession #: P48061
TARC (CCL17)
  • Gene Symbol: CCL17
  • Gene ID: 6361
  • Accession #: Q92583
TECK (CCL25)
  • Gene Symbol: CCL25
  • Gene ID: 6370
  • Accession #: O15444
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human Chemokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product Type:
Protein Array
Analytes:
BLC (CXCL13)
Analytes:
CCL28 (MEC)
Analytes:
Ck beta 8-1 (CCL23)
Analytes:
CTACK (CCL27)
Analytes:
CXCL16
Analytes:
ENA-78 (CXCL5)
Analytes:
Eotaxin-1 (CCL11)
Analytes:
Eotaxin-2 (MPIF-2/CCL24)
Analytes:
Eotaxin-3 (CCL26)
Analytes:
Fractalkine (CX3CL1)
Analytes:
GCP-2 (CXCL6)
Analytes:
GRO alpha/beta/gamma
Analytes:
GRO alpha (CXCL1)
Analytes:
HCC-4 (CCL16)
Analytes:
I-309 (TCA-3/CCL1)
Analytes:
I-TAC (CXCL11)
Analytes:
IL-8 (CXCL8)
Analytes:
IP-10 (CXCL10)
Analytes:
Lymphotactin (XCL1)
Analytes:
MCP-1 (CCL2)
Analytes:
MCP-2 (CCL8)
Analytes:
MCP-3 (MARC/CCL7)
Analytes:
MCP-4 (CCL13)
Analytes:
MDC (CCL22)
Analytes:
MIG (CXCL9)
Analytes:
MIP-1 alpha (CCL3)
Analytes:
MIP-1 beta (CCL4)
Analytes:
MIP-1 delta (CCL15)
Analytes:
MIP-3 alpha (CCL20)
Analytes:
MIP-3 beta (CCL19)
Analytes:
MPIF-1 (CCL23)
Analytes:
NAP-2 (PPBP/CXCL7)
Analytes:
PARC (CCL18)
Analytes:
RANTES (CCL5)
Analytes:
SDF-1 alpha (CXCL12 alpha)
Analytes:
SDF-1 beta (CXCL12 beta)
Analytes:
TARC (CCL17)
Analytes:
TECK (CCL25)
Reactivity:
Human
Applications:
Multiplex Array
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