Human CENPH Recombinant Protein (RPPB3146)

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SKU:
RPPB3146
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9H3R5

Description

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Product Name:Human CENPH Recombinant Protein
Product Code:RPPB3146
Size:20µg
Species:Human
Target:CENPH
Synonyms:Centromere protein H, Interphase centromere complex protein 35, CENP-H, NNF1, PMF1, ICEN35, Kinetochore protein CENP-H.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The CENPH solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MLNKLIMKSQ QESWDLEEKL LDIRKKRLQL KQASESKLLE IQTEKNKQKI DLDSMENSER IKIIRQNLQM EIKITTVIQH VFQNLILGSK VNWAEDPALK EIVLQLEKNV DMM

CENPH is a member of the centromere protein H family. CENPH protein is a component of the CENPA-NAC (nucleosome-associated) complex which has a vital part in assembly of kinetochore proteins. The CENPA-NAC complex utilizes the CENPA-CAD (nucleosome distal) complex and is involved in integration of freshly synthesized CENPA into centromeres.

CENPH Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 133 amino acids (136-247) and having a molecular mass of 15.5 kDa.The CENPH is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:CENPH: Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate. Belongs to the centromere protein H family.
UniProt Protein Details:

Protein type:Cell cycle regulation

Chromosomal Location of Human Ortholog: 5p15.2

Cellular Component: cytosol; kinetochore; nucleoplasm; nucleus

Molecular Function:kinetochore binding; protein binding

Biological Process: DNA replication-independent nucleosome assembly at centromere; kinetochore organization and biogenesis; sister chromatid cohesion

NCBI Summary:Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. [provided by RefSeq, Jul 2008]
UniProt Code:Q9H3R5
NCBI GenInfo Identifier:74733576
NCBI Gene ID:64946
NCBI Accession:Q9H3R5.1
UniProt Secondary Accession:Q9H3R5,A8K3Y1,
UniProt Related Accession:Q9H3R5
Molecular Weight:28,481 Da
NCBI Full Name:Centromere protein H
NCBI Synonym Full Names:centromere protein H
NCBI Official Symbol:CENPH��
NCBI Protein Information:centromere protein H
UniProt Protein Name:Centromere protein H
UniProt Synonym Protein Names:Interphase centromere complex protein 35
UniProt Gene Name:CENPH��
UniProt Entry Name:CENPH_HUMAN
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