Neurotrophins Recombinant Proteins

Human CDNF Recombinant Protein (RPPB0123)

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RPPB0123

Description

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Product Name:Human CDNF Recombinant Protein
Product Code:RPPB0123
Size:20µg
Species:Human
Target:CDNF
Synonyms:Cerebral neurotrophic factor, arginine-rich, mutated in early stage tumors-like 1, Conserved neurotrophic factor, ARMET-like protein 1, ARMETL1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:CDNF protein was lyophilized from a 0.2�m filtered concentrated solution in 1xPBS, pH 7.4.
Solubility:It is recommended to reconstitute the lyophilized�CDNF in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized CDNF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CDNF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity:Greater than 96.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:QEAGGRPGAD CEVCKEFLNR FYKSLIDRGV NFSLDTIEKE LISFCLDTKG KENRLCYYLG ATKDAATKIL SEVTRPMSVH MPAMKICEKL KKLDSQICEL KYEKTLDLAS VDLRKMRVAE LKQILHSWGE ECRACAEKTD YVNLIQELAP KYAATHPKTE L
Biological Activity:The ED50 as determined by its ability to stimulate the proliferation of rat C6 cells is 15-25�g/ml, corresponding to a specific activity of 40-67units/mg.

CDNF is a member of the ARMET family and acts as a trophic factor for neurons. CDNF inhibits the (6-OHDA)-induced degeneration of neurons. When CDNF controlled after 6-OHDA-lesioning, it reestablishes the function and inhibits the degeneration of neurons in substantia nigra. CDNF is universally expressed in neuronal and non-neuronal tissues. The highest levels in the brain are found in the optic nerve and corpus callosum.

CDNF Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 161 amino acids and having a molecular mass of 18.5kDa.The CDNF is purified by proprietary chromatographic techniques.

UniProt Protein Function:ARMETL1: Trophic factor for dopamine neurons. Prevents the 6- hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. When administered after 6-OHDA-lesioning, restores the dopaminergic function and prevents the degeneration of dopaminergic neurons in substantia nigra. Belongs to the ARMET family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: extracellular region

Molecular Function:growth factor activity

UniProt Code:Q49AH0
NCBI GenInfo Identifier:143955278
NCBI Gene ID:441549
NCBI Accession:Q49AH0.2
UniProt Related Accession:Q49AH0
Molecular Weight:
NCBI Full Name:Cerebral dopamine neurotrophic factor
NCBI Synonym Full Names:cerebral dopamine neurotrophic factor
NCBI Official Symbol:CDNF��
NCBI Official Synonym Symbols:ARMETL1��
NCBI Protein Information:cerebral dopamine neurotrophic factor
UniProt Protein Name:Cerebral dopamine neurotrophic factor
UniProt Synonym Protein Names:ARMET-like protein 1; Conserved dopamine neurotrophic factor
Protein Family:Cerebral dopamine neurotrophic factor
UniProt Gene Name:CDNF��
UniProt Entry Name:CDNF_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q49AH0
Research Area:
Neurotrophins
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