Human CDH5 Recombinant Protein (RPPB3128)
- SKU:
- RPPB3128
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P33151
Description
| Product Name: | Human CDH5 Recombinant Protein |
| Product Code: | RPPB3128 |
| Size: | 10µg |
| Species: | Human |
| Target: | CDH5 |
| Synonyms: | Cadherin 5, VE-Cadherin, Cadherin 5 Type 2 VE-Cadherin (Vascular Epithelium), CDH5, Cadherin 5 Type 2 (Vascular Endothelium), Vascular Endothelial Cadherin, 7B4 Antigen, Endothelial-Specific Cadherin, Cd144 Antigen, CD144 Antigen, Cadherin-5, CD144, 7B4. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterilefiltered colorless solution. |
| Formulation: | CDH5 solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) & 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greaterthan 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | NPAQRDTHSL LPTHRRQKRD WIWNQMHIDE EKNTSLPHHV GKIKSSVSRK NAKYLLKGEY VGKVFRVDAE TGDVFAIERL DRENISEYHL TAVIVDKDTG ENLETPSSFT IKVHDVNDNW PVFTHRLFNA SVPESSAVGT SVISVTAVDA DDPTVGDHAS VMYQILKGKE YFAIDNSGRI ITITKSLDRE KQARYEIVVE ARDAQGLRGD SGTATVLVTL QDINDNFPFF TQTKYTFVVP EDTRVGTSVG SLFVEDPDEP QNRMTKYSIL RGDYQDAFTI ETNPAHNEGI IKPMKPLDYE YIQQYSFIVE ATDPTIDLRY MSPPAGNRAQ VIINITDVDE PPIFQQPFYH FQLKENQKKP LIGTVLAMDP DAARHSIGYS IRRTSDKGQF FRVTKKGDIY NEKELDREVY PWYNLTVEAK ELDSTGTPTG KESIVQVHIE VLDENDNAPE FAKPYQPKVC ENAVHGQLVL QISAIDKDIT PRNVKFKFIL NTENNFTLTD NHDNTANITV KYGQFDREHT KVHFLPVVIS DNGMPSRTGT STLTVAVCKC NEQGEFTFCE DMAAQVGVSI QLEPKSCDKT HTCPPCPAPE LLGGPSVFLF PPKPKDTLMI SRTPEVTCVV VDVSHEDPEV KFNWYVDGVE VHNAKTKPRE EQYNSTYRVV SVLTVLHQDW LNGKEYKCKV SNKALPAPIE KTISKAKGQP REPQVYTLPP SRDELTKNQV SLTCLVKGFY PSDIAVEWES NGQPENNYKT TPPVLDSDGS FFLYSKLTVD KSRWQQGNVF SCSVMHEALH NHYTQKSLSL SPGKHHHHHH |
Cadherin-5 (CDH5) belongs to the atypical/type 2 subgroup of Cadherin homophilic adhesion proteins. Cadherin-5 is a calcium-dependent cell-cell adhesion molecule, which is comprised of 5 extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Cadherin-5 protein plays a role in the formation, maturation and remodeling of the vascular wall. CDH5 is generally considered to be specific for vascular endothelia in which it is either the sole or the predominant cadherin, often co-existing with N-cadherin. Moreover, the CDH5 protein regulates or is regulated by VEGF R2, type 1 and type 2 TGF-beta receptors, and other endothelial junction proteins such as JAM-C, Claudin-5, and N-Cadherin. CDH5 also connects with alpha-catenin forming a link to the cytoskeleton. CDH5 functions jointly with KRIT1 to create and maintain correct endothelial cell polarity and vascular lumen. Furthermore, Cadherin-5 is essential for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. The CDH5 gene is located in a gene cluster in a region on the long arm of chromosome 16 which is involved in loss of heterozygosity events in breast and prostate cancer.
Cadherin 5 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 810 amino acids (29-599 a.a.) and having a molecular mass of 91.5kDa (Migrates at 70-100kDa on SDS-PAGE under reducing conditions). CDH5 is expressed with a 239 amino acid hIgG-His-tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CDH5: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. Interacts via cadherin 5 domain with PTPRB. Interacts with TRPC4. Interacts with KRIT1. Endothelial tissues and brain. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral Chromosomal Location of Human Ortholog: 16q22.1 Cellular Component: adherens junction; tight junction; membrane; integral to membrane; plasma membrane; intercellular junction; cell junction Molecular Function:protein binding; beta-catenin binding; calcium ion binding; protein phosphatase binding; receptor binding Biological Process: intercellular junction assembly and maintenance; negative regulation of cell proliferation; vascular endothelial growth factor receptor signaling pathway; homophilic cell adhesion; blood vessel maturation |
| NCBI Summary: | This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P33151 |
| NCBI GenInfo Identifier: | 322510142 |
| NCBI Gene ID: | 1003 |
| NCBI Accession: | P33151.5 |
| UniProt Secondary Accession: | P33151,Q4VAI5, Q4VAI6, |
| UniProt Related Accession: | P33151 |
| Molecular Weight: | |
| NCBI Full Name: | Cadherin-5 |
| NCBI Synonym Full Names: | cadherin 5, type 2 (vascular endothelium) |
| NCBI Official Symbol: | CDH5�� |
| NCBI Official Synonym Symbols: | 7B4; CD144�� |
| NCBI Protein Information: | cadherin-5; 7B4 antigen; VE-cadherin; cd144 antigen; endothelial-specific cadherin; vascular endothelial cadherin; cadherin 5, type 2, VE-cadherin (vascular epithelium) |
| UniProt Protein Name: | Cadherin-5 |
| UniProt Synonym Protein Names: | 7B4 antigen; Vascular endothelial cadherin; VE-cadherin; CD_antigen: CD144 |
| Protein Family: | Cadherin |
| UniProt Gene Name: | CDH5�� |
| UniProt Entry Name: | CADH5_HUMAN |
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