Human CDC34 Recombinant Protein (RPPB1482)
- SKU:
- RPPB1482
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P49427
Description
| Product Name: | Human CDC34 Recombinant Protein |
| Product Code: | RPPB1482 |
| Size: | 20µg |
| Species: | Human |
| Target: | CDC34 |
| Synonyms: | UB2R1, CDC-34, Ubiquitin-conjugating enzyme E2 R1, Ubiquitin-protein ligase R1, Ubiquitin-conjugating enzyme E2-32 kDa complementing, E2-CDC34, CDC34, EC 6.3.2.19, UBC3, UBE2R1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The CDC34 (1mg/ml) protein solution contains 20mM Tris-Hcl pH-8, 10% glycerol, 50mM NaCl and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MARPLVPSSQ KALLLELKGL QEEPVEGFRV TLVDEGDLYN WEVAIFGPPN TYYEGGYFKA RLKFPIDYPY SPPAFRFLTK MWHPNIYETG DVCISILHPP VDDPQSGELP SERWNPTQNV RTILLSVISL LNEPNTFSPA NVDASVMYRK WKESKGKDRE YTDIIRKQVL GTKVDAERDG VKVPTTLAEY CVKTKAPAPD EGSDLFYDDY YEDGEVEEEA DSCFGDDEDD SGTEES |
CDC34 is important in the control of cell cycle and DNA replication. Cdc34 in association with different E3 complexes, including SCF, has been shown to target many different substrates for ubiquitination and degradation during cell division, signal transduction, and development. Cdc34 substrates that have been characterized include IkB, B-Myb, Wee1, MyoD, ATF5, p27Xic1, and p27Kip1. Additionally, substrates such as b-catenin, p21Cip1, E2F, cyclin E, and cyclin D are putative substrates of Cdc34 by virtue of their SCF requirement for proteolysis. Cdc34 has been demonstrated to self-associate through a domain in the C-terminus, and is phosphorylated and ubiquitinylated in vivo. This protein is useful for in vitro ubiquitinylation reactions.
CDC34 Human Recombinant protein fused to a 20 amino acid N-terminal His-Tag produced in E.Coli is a single, non-glycosylated polypeptide chain containing 256 amino acids (1-236 a.a.) and having a molecular mass of 28.9 kDa.�CDC34�is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CDC34: a member of the ubiquitin-conjugating enzyme family. Is a part of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which is required for ubiquitin-mediated degradation of cell cycle G1 regulators. Required for the initiation of DNA replication. |
| UniProt Protein Details: | Protein type:Ligase; Ubiquitin ligase; EC 6.3.2.19; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:ATP binding; protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity Biological Process: DNA replication initiation; G1/S transition of mitotic cell cycle; positive regulation of neuron apoptosis; proteasomal ubiquitin-dependent protein catabolic process; protein modification process; protein polyubiquitination; protein ubiquitination |
| NCBI Summary: | The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P49427 |
| NCBI GenInfo Identifier: | 2507505 |
| NCBI Gene ID: | 997 |
| NCBI Accession: | P49427.2 |
| UniProt Secondary Accession: | P49427,A8K689, |
| UniProt Related Accession: | P49427 |
| Molecular Weight: | 26,737 Da |
| NCBI Full Name: | Ubiquitin-conjugating enzyme E2 R1 |
| NCBI Synonym Full Names: | cell division cycle 34 |
| NCBI Official Symbol: | CDC34�� |
| NCBI Official Synonym Symbols: | UBC3; UBCH3; UBE2R1; E2-CDC34�� |
| NCBI Protein Information: | ubiquitin-conjugating enzyme E2 R1 |
| UniProt Protein Name: | Ubiquitin-conjugating enzyme E2 R1 |
| UniProt Synonym Protein Names: | (E3-independent) E2 ubiquitin-conjugating enzyme R1 (EC:2.3.2.24 |
| Protein Family: | Ubiquitin-conjugating enzyme |
| UniProt Gene Name: | CDC34�� |
| UniProt Entry Name: | UB2R1_HUMAN |
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