Human CD86/B7-2 PharmaGenie ELISA Kit (SBRS0401)

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SKU:
SBRS0401
Product Type:
ELISA Kit
Reactivity:
Human
Applications:
ELISA
ELISA Type:
Sandwich

Description

system_update_altDatasheet

Human CD86/B7-2 PharmaGenie ELISA Kit (SBRS0401)

Product SKU:SBRS0401
Size:96T
Application:The antibody pair provided in this kit recognizes Human CD86 (B7-2).
Uniprot:P42081
Gene ID:942
Gene Names:CD28LG2 / CD86
Synonyms:T-lymphocyte activation antigen CD86 (Activation B7-2 antigen) (B70) (BU63) (CTLA-4 counter-receptor B7.2) (FUN-1) (CD antigen CD86)
Target Species:Human
Compatible Sample Types:Cell Culture Supernatants, Plasma, Serum
Design Principle:Sandwich-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Quantitative
Range:0.82 ng/ml - 200 ng/ml
Sensitivity:0.82 ng/ml
Recommended Dilution:2 fold
Storage/Stability:May be stored for up to 6 months at 2 to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2 to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
  1. Pre-Coated 96-well Strip Microplate
  2. Wash Buffer
  3. Stop Solution
  4. Assay Diluent(s)
  5. Lyophilized Standard
  6. Biotinylated Detection Antibody
  7. Streptavidin-Conjugated HRP
  8. TMB One-Step Substrate

Other materials and equipment required:

The Assay Genie Human CD86/B7-2 PharmaGenie ELISA Kit (SBRS0401) will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • Precision pipettes to deliver 2 ul to 1 ul volumes
  • Adjustable 1-25 ul pipettes for reagent preparation
  • 100 ul and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 ul of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 ul of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 ul of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 ul of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 ul of Stop Solution to each well.
  11. Read at 450 nm immediately.
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