Human CD50/ICAM-3 Recombinant Protein (RPES4223)
- SKU:
- RPES4223
- Product Type:
- Recombinant Protein
- Species:
- Human
Description
Human CD50/ICAM-3 Recombinant Protein
The protein ICAM-3, also known as CD50, is a member of the intercellular adhesion molecule (ICAM) family consisting three members. It is a DC-SIGN ligand that is constitutively expressed on resting leukocytes, and is thus an important molecule for the first immune response. ICAM-3 comprises of five immunoglobulin-like domains, and binds LFA-1 through its two N-terminal domains. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM-3 binds to LFA-1 on antigen-presenting cells (APC) stabilizing the T cell-APC interaction, facilitating signaling through the CD3/TCR complex. However, recent evidence using cultured and transformed T cells suggests ICAM-3 may also function in signaling. It has been reported that CD50 molecule can play a role in developing functionally mature T lymphocytes and its expression increases during the maturation process of T lymphocytes. In addition, the interactions of ICAM-3 and LFA-1 facilitate HIV-1- induced virological synapse formation between T cells. ICAM-3 is associated with an increase of cellular radio-resistance and cancer cell proliferation. It could be considered as a candidate for anti-cancer drug development and as a cancer diagnostic marker.
| Product Name: | Human CD50/ICAM-3 Recombinant Protein (RPES4223) |
| Product Code: | RPES4223 |
| Size: | 20µg |
| Species: | Human |
| Expressed Host: | HEK293 Cells |
| Synonyms: | CD50,CDW50,ICAM-3,ICAM-R |
| Accession: | NP_002153.2 |
| Sequence: | Met 1-His 485 |
| Fusion tag: | C-His |
| Activity: | Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.When cells are added to ICAM3-coated plates (12.5 µg/mL , 100 ?L/well), approximately >25% cells will adhere specifically. |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | A DNA sequence encoding the extracellular domain of human ICAM3 (NP_002153.2) (Met 1-His 485) was expressed, with the C-terminal fused polyhistidine tag. |
| Purity: | > 92 % as determined by reducing SDS-PAGE. |
| Mol Mass: | 50.8 kDa |
| AP Mol Mass: | 100-120 kDa |
| Formulation: | Lyophilized from sterile PBS, pH 7.5 |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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