Description
| Product Name: | Human CD43 Recombinant Protein |
| Product Code: | RPPB3074 |
| Size: | 20µg |
| Species: | Human |
| Target: | CD43 |
| Synonyms: | Leukosialin, Galactoglycoprotein, GALGP, Leukocyte sialoglycoprotein, Sialophorin, SPN, CD43, leukosialin precursor, GPL115, LSN. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | CD43 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSSTTAVQT PTSGEPLVST SEPLSSKMYT TSITSDPKAD STGDQTSALP PSTSINEGSP LWTSIGASTG SPLPEPTTYQ EVSIKMSSVP QETPHATSHP AVPITANSLG SHTVTGGTIT TNSPETSSRT SGAPVTTAAS SLETSRGTSG PPLTMATVSL ETSKGTSGPP VTMATDSLET STGTTGPPVT MTTGSLEPSS GASGPQVSSV KLSTMMSPTT STNASTVPFR NPDENSR |
CD43 is a key sialoglycoprotein located in the surface of T lymphocytes, monocytes, granulocytes, thymocytes and some B lymphocytes. CD43 is a part of a physiologic ligand-receptor complex which participates in T-cell activation. Throughout T-cell activation, CD43 is actively taken out from the T-cell-APC (antigen-presenting cell) contact site, signifying CD43 �has a negative regulatory role in adaptive immune response.�
CD43 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 257 amino acids (20-253a.a.) and having a molecular mass of 25.8kDa.CD43 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | sialophorin: One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1. During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response. Interacts with HIPK2 via the cytoplasmic domain. Interacts with RDX. Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas. |
| UniProt Protein Details: | Protein type:Cell surface; Membrane protein, integral Chromosomal Location of Human Ortholog: 16p11.2 Cellular Component: extracellular space; cell surface; membrane; integral to plasma membrane; plasma membrane; basement membrane; uropod; external side of plasma membrane Molecular Function:protein binding; transmembrane receptor activity Biological Process: positive regulation of tumor necrosis factor biosynthetic process; regulation of defense response to virus; establishment and/or maintenance of cell polarity; negative regulation of cell adhesion; chemotaxis; signal transduction; negative thymic T cell selection; negative regulation of T cell proliferation; cell surface receptor linked signal transduction; response to protozoan; T cell costimulation; defense response to bacterium; cellular defense response; positive regulation of T cell proliferation; immune response; positive regulation of protein amino acid phosphorylation; blood coagulation; negative regulation of type IV hypersensitivity; leukocyte migration |
| NCBI Summary: | The protein encoded by this gene is a major sialoglycoprotein found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It may be part of a physiologic ligand-receptor complex involved in T-cell activation. During T-cell activation, this protein is actively removed from the T-cell-APC (antigen-presenting cell) contact site, suggesting a negative regulatory role in adaptive immune response. [provided by RefSeq, Sep 2011] |
| UniProt Code: | P16150 |
| NCBI GenInfo Identifier: | 126213 |
| NCBI Gene ID: | 6693 |
| NCBI Accession: | P16150.1 |
| UniProt Secondary Accession: | P16150,A8K9B1, |
| UniProt Related Accession: | P16150 |
| Molecular Weight: | |
| NCBI Full Name: | Leukosialin |
| NCBI Synonym Full Names: | sialophorin |
| NCBI Official Symbol: | SPN�� |
| NCBI Official Synonym Symbols: | LSN; CD43; GALGP; GPL115�� |
| NCBI Protein Information: | leukosialin; galactoglycoprotein; leukocyte sialoglycoprotein; sialophorin (gpL115, leukosialin, CD43) |
| UniProt Protein Name: | Leukosialin |
| UniProt Synonym Protein Names: | Galactoglycoprotein; GALGP; Leukocyte sialoglycoprotein; Sialophorin; CD_antigen: CD43 |
| Protein Family: | Leukosialin |
| UniProt Gene Name: | SPN�� |
| UniProt Entry Name: | LEUK_HUMAN |
