CD Antigens Recombinant Proteins

Human CD274 Recombinant Protein (RPPB3054)

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RPPB3054

Description

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Product Name:Human CD274 Recombinant Protein
Product Code:RPPB3054
Size:10µg
Species:Human
Target:CD274
Synonyms:CD274 molecule, CD274 antigen, programmed cell death 1 ligand 1, PD-L1, PDCD1L1, PDCD1LG1, PDCD1 ligand 1, B7 homolog 1, B7H1.
Source:Sf9 Insect cells
Physical Appearance:Sterile Filtered colorless solution.
Formulation:CD274 protein solution (1mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:ADLFTVTVPK DLYVVEYGSN MTIECKFPVE KQLDLAALIV YWEMEDKNII QFVHGEEDLK VQHSSYRQRA RLLKDQLSLG NAALQITDVK LQDAGVYRCM ISYGGADYKR ITVKVNAPYN KINQRILVVD PVTSEHELTC QAEGYPKAEV IWTSSDHQVL SGKTTTTNSK REEKLFNVTS TLRINTTTNE IFYCTFRRLD PEENHTAELV IPELPLAHPP NERLEPKSCD KTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSRDELTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGKHHHH HH

CD274 takes part in the costimulatory signal, vital for T-cell proliferation and creation of IL10 and IFNG, in a PDCD1-independent and an IL2-dependent manner. CD274 collaboration with PDCD1 inhibits cytokine production and T-cell proliferation.

CD274 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 462 amino acids (19-238a.a.) and having a molecular mass of 52.5kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). CD274 is expressed with a 239 amino acids hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:Involved in the costimulatory signal essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.
NCBI Summary:The protein encoded by this gene is an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Mice deficient for this gene display a variety of phenotypes including decreased allogeneic fetal survival rates and severe experimental autoimmune encephalomyelitis. [provided by RefSeq, Sep 2015]
UniProt Code:Q9EP73
NCBI GenInfo Identifier:83287885
NCBI Gene ID:60533
NCBI Accession:Q9EP73.1
UniProt Related Accession:Q9EP73
Molecular Weight:32,780 Da
NCBI Full Name:Programmed cell death 1 ligand 1
NCBI Synonym Full Names:CD274 antigen
NCBI Official Symbol:Cd274��
NCBI Official Synonym Symbols:B7h1; Pdl1; Pdcd1l1; Pdcd1lg1; A530045L16Rik��
NCBI Protein Information:programmed cell death 1 ligand 1
UniProt Protein Name:Programmed cell death 1 ligand 1
UniProt Synonym Protein Names:B7 homolog 1; B7-H1; CD_antigen: CD274
Protein Family:Programmed cell death
UniProt Gene Name:Cd274��
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9EP73
Research Area:
CD Antigens
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