Description
| Product Name: | Human CBR4 Recombinant Protein |
| Product Code: | RPPB1477 |
| Size: | 10µg |
| Species: | Human |
| Target: | CBR4 |
| Synonyms: | Carbonyl reductase family member 4, 3-oxoacyl-[acyl-carrier-protein] reductase, Quinone reductase CBR4, CBR4, SDR45C1, FLJ14431. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The CBR4 solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 5mM DTT and 200mM NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MDKVCAVFGG SRGIGRAVAQ LMARKGYRLA VIARNLEGAK AAAGDLGGDH LAFSCDVAKE HDVQNTFEEM EKHLGRVNFL VNAAGINRDG LLVRTKTEDM VSQLHTNLLG SMLTCKAAMR TMIQQQGGSI VNVGSIVGLK GNSGQSVYSA SKGGLVGFSR ALAKEVARKK IRVNVVAPGF VHTDMTKDLK EEHLKKNIPL GRFGETIEVA HAVVFLLESP YITGHVLVVD GGLQLIL |
CBR4 is a member of the short-chain dehydrogenase/reductase family. CBR4 has a role in biosynthesis of fatty acids in the mitochondria and a broad substrate specificity and reduces 9,10-phenanthrenequinone, 1,4-benzoquinone and a range of other o-quinones and p-quinones (in vitro). CBR4 formation of a heteroteramer with HSD17B8 has NADH-dependent 3-ketoacyl-acyl carrier protein reductase activity for o- and p-quinones.
CBR4 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 257 amino acids (1-237 a.a.) and having a molecular mass of 27.5kDa. The CBR4 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CBR4: The heteroteramer with HSD17B8 has NADH-dependent 3- ketoacyl-acyl carrier protein reductase activity. May play a role in biosynthesis of fatty acids in mitochondria. The homotetramer may act as NADPH-dependent quinone reductase. Has broad substrate specificity and reduces 9,10-phenanthrenequinone, 1,4-benzoquinone and various other o-quinones and p-quinones (in vitro). Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 1.-.-.-; Oxidoreductase; Mitochondrial; EC 1.1.1.- Chromosomal Location of Human Ortholog: 4q32.3 Cellular Component: mitochondrial matrix Molecular Function:protein binding; NAD(P)H dehydrogenase (quinone) activity; NADPH dehydrogenase (quinone) activity; quinone binding Biological Process: fatty acid biosynthetic process; protein homotetramerization |
| UniProt Code: | Q8N4T8 |
| NCBI GenInfo Identifier: | 269849708 |
| NCBI Gene ID: | 84869 |
| NCBI Accession: | Q8N4T8.3 |
| UniProt Secondary Accession: | Q8N4T8,Q8WTW8, Q96K93, |
| UniProt Related Accession: | Q8N4T8 |
| Molecular Weight: | 237 |
| NCBI Full Name: | Carbonyl reductase family member 4 |
| NCBI Synonym Full Names: | carbonyl reductase 4 |
| NCBI Official Symbol: | CBR4�� |
| NCBI Official Synonym Symbols: | SDR45C1�� |
| NCBI Protein Information: | carbonyl reductase family member 4; carbonic reductase 4; quinone reductase CBR4; 3-oxoacyl-[acyl-carrier-protein] reductase; short chain dehydrogenase/reductase family 45C, member 1 |
| UniProt Protein Name: | Carbonyl reductase family member 4 |
| UniProt Synonym Protein Names: | 3-oxoacyl-[acyl-carrier-protein] reductase (EC:1.1.1.-); Quinone reductase CBR4 |
| Protein Family: | Carbonyl reductase family |
| UniProt Gene Name: | CBR4�� |
| UniProt Entry Name: | CBR4_HUMAN |
