CASP8 is associated with apoptosis, also involved in other processes, like inflammation and immunity. Diseases associated with CASP8 include Caspase 8 Deficiency and Hepatocellular Carcinoma. Pathways associated with CASP8 include Activated TLR4 signalling and Nanoparticle triggered regulated necrosis. Gene Ontology (GO) annotations related to this gene include protein heterodimerization activity and protein-containing complex binding. An important paralog of CASP8 is CASP10. The Assay Genie Human Caspase-8 ELISA is a highly sensitive assay for the quantitative measurement of Caspase-8 in serum, blood, plasma, cell culture supernatant and tissue samples.
This immunoassay kit allows for the in vitro quantitative determination of Human CASP8 concentrations in serum plasma and other biological fluids.
Sensitivity:
0.094ng/ml
Range:
0.156-10ng/ml
Storage:
4°C for 6 months
Note:
For Research Use Only
Recovery:
Matrices listed below were spiked with certain level of Human CASP8 and the recovery rates were calculated by comparing the measured value to the expected amount of Human CASP8 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-103
97
EDTA plasma(n=5)
85-96
90
UFH plasma(n=5)
87-98
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human CASP8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
91-103%
87-98%
86-105%
EDTA plasma(n=5)
85-98%
82-101%
82-94%
UFH plasma(n=5)
83-100%
80-96%
83-99%
CV(%):
Intra-Assay: CV<8% Inter-Assay: CV<10%
Component
Quantity
Storage
ELISA Microplate (Dismountable)
8×12 strips
4°C for 6 months
Lyophilized Standard
2
4°C/-20°C
Sample/Standard Dilution Buffer
20ml
4°C
Biotin-labeled Antibody(Concentrated)
120ul
4°C (Protect from light)
Antibody Dilution Buffer
10ml
4°C
HRP-Streptavidin Conjugate(SABC)
120ul
4°C (Protect from light)
SABC Dilution Buffer
10ml
4°C
TMB Substrate
10ml
4°C (Protect from light)
Stop Solution
10ml
4°C
Wash Buffer(25X)
30ml
4°C
Plate Sealer
5
-
Other materials and equipment required:
Microplate reader with 450 nm wavelength filter
Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
CASP8: Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death- inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp--AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex. Heterotetramer that consists of two anti-parall
Fill out our quote form below and a dedicated member of staff will get back to you within one working day!
