CASP7 (Caspase 7) encoded protein belongs to the caspase family and is involved in the apoptotic process. CASP7 is overexpressed in many tumors, making it a potential target for cancer therapy. Additionally, mutations in CASP7 have been associated with limb-girdle muscular dystrophy 2D, a muscle wasting disorder. The encoded protein is known to interact with Bcl-2, an anti-apoptotic protein, and to be involved in the mitochondrial pathway of apoptosis. n important paralog of CASP7 is CASP3.
This immunoassay kit allows for the in vitro quantitative determination of Human CASP7 concentrations in serum plasma and other biological fluids.
Sensitivity:
0.188ng/ml
Range:
0.313-20ng/ml
Storage:
4°C for 6 months
Note:
For Research Use Only
Recovery:
Matrices listed below were spiked with certain level of Human CASP7 and the recovery rates were calculated by comparing the measured value to the expected amount of Human CASP7 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-97
92
EDTA plasma(n=5)
85-100
91
UFH plasma(n=5)
87-105
99
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human CASP7 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-102%
87-104%
91-105%
EDTA plasma(n=5)
87-101%
82-92%
85-100%
UFH plasma(n=5)
87-95%
83-93%
89-98%
CV(%):
Intra-Assay: CV<8% Inter-Assay: CV<10%
Component
Quantity
Storage
ELISA Microplate (Dismountable)
8×12 strips
4°C for 6 months
Lyophilized Standard
2
4°C/-20°C
Sample/Standard Dilution Buffer
20ml
4°C
Biotin-labeled Antibody(Concentrated)
120ul
4°C (Protect from light)
Antibody Dilution Buffer
10ml
4°C
HRP-Streptavidin Conjugate(SABC)
120ul
4°C (Protect from light)
SABC Dilution Buffer
10ml
4°C
TMB Substrate
10ml
4°C (Protect from light)
Stop Solution
10ml
4°C
Wash Buffer(25X)
30ml
4°C
Plate Sealer
5
-
Other materials and equipment required:
Microplate reader with 450 nm wavelength filter
Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
CASP7: Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp--Gly- 217' bond. Overexpression promotes programmed cell death. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 11 kDa (p11) subunit. Interacts with BIRC6/bruce. Highly expressed in lung, sk
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