Human Caspase 3 ELISA Kit
- SKU:
- HUFI00393
Description
system_update_altDatasheet - तकनीकी मैनुअलsystem_update_altMSDS - तकनीकी मैनुअल
Human Caspase 3 ELISA Kit
Caspase 3 is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. Caspase 3 cleaves and inactivates poly(ADP-ribose) polymerase. Caspase 3 is involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. The Assay Genie Human Caspase 3 ELISA Kit is a highly sensitive assay for the quantitative measurement of Caspase 3 in serum, blood, plasma, cell culture supernatant and tissue samples.
| Product Name: | Human Caspase 3 ELISA Kit |
| Product Code: | HUFI00393 |
| Size: | 96 Assays |
| Alias: | CASP3, CPP32, CPP32B, SCA-1, Apoptain, Yama, Apopain, LICE-1, YAMA, Apopain, apoptosis-related cysteine protease, CASP-3, caspase 3, apoptosis-related cysteine peptidase, caspase-3, CPP-32, CPP32, SREBP cleavage activity 1, Cysteine protease CPP32, PARP cleavage protease, procaspase3, Protein Yama |
| Detection method: | Sandwich ELISA, Double Antibody |
| Application: | This immunoassay kit allows for the in vitro quantitative determination of Human CASP3 concentrations in serum plasma and other biological fluids. |
| Sensitivity: | 0.188ng/ml |
| Range: | 0.313-20ng/ml |
| Storage: | 4°C for 6 months |
| Note: | For Research Use Only |
| Recovery: | Matrices listed below were spiked with certain level of Human CASP3 and the recovery rates were calculated by comparing the measured value to the expected amount of Human CASP3 in samples. | ||||||||||||||||
| |||||||||||||||||
| Linearity: | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human CASP3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. | ||||||||||||||||
| |||||||||||||||||
| CV(%): | Intra-Assay: CV<8% Inter-Assay: CV<10% |
| Component | Quantity | Storage |
| ELISA Microplate (Dismountable) | 8×12 strips | 4°C for 6 months |
| Lyophilized Standard | 2 | 4°C/-20°C |
| Sample/Standard Dilution Buffer | 20ml | 4°C |
| Biotin-labeled Antibody(Concentrated) | 120ul | 4°C (Protect from light) |
| Antibody Dilution Buffer | 10ml | 4°C |
| HRP-Streptavidin Conjugate(SABC) | 120ul | 4°C (Protect from light) |
| SABC Dilution Buffer | 10ml | 4°C |
| TMB Substrate | 10ml | 4°C (Protect from light) |
| Stop Solution | 10ml | 4°C |
| Wash Buffer(25X) | 30ml | 4°C |
| Plate Sealer | 5 | - |
Other materials and equipment required:
- Microplate reader with 450 nm wavelength filter
- Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
- Incubator
- Deionized or distilled water
- Absorbent paper
- Buffer resevoir
| Uniprot | P42574 |
| UniProt Protein Function: | CASP3: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp--Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of Fill out our quote form below and a dedicated member of staff will get back to you within one working day! Additional Information
Related Products   Human Caspase-3 PharmaGenie ELISA Kit (SBRS0348)
system_update_altDatasheetHuman Caspase-3 PharmaGenie ELISA Kit (SBRS0348)Overview
 |
