Caspase 3 is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. Caspase 3 cleaves and inactivates poly(ADP-ribose) polymerase. Caspase 3 is involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. The Assay Genie Human Caspase 3 ELISA Kit is a highly sensitive assay for the quantitative measurement of Caspase 3 in serum, blood, plasma, cell culture supernatant and tissue samples.
This immunoassay kit allows for the in vitro quantitative determination of Human CASP3 concentrations in serum plasma and other biological fluids.
Sensitivity:
0.188ng/ml
Range:
0.313-20ng/ml
Storage:
4°C for 6 months
Note:
For Research Use Only
Recovery:
Matrices listed below were spiked with certain level of Human CASP3 and the recovery rates were calculated by comparing the measured value to the expected amount of Human CASP3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-101
94
EDTA plasma(n=5)
90-103
97
UFH plasma(n=5)
91-105
98
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human CASP3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-103%
85-99%
86-94%
EDTA plasma(n=5)
82-97%
84-96%
83-99%
UFH plasma(n=5)
83-98%
84-98%
81-99%
CV(%):
Intra-Assay: CV<8% Inter-Assay: CV<10%
Component
Quantity
Storage
ELISA Microplate (Dismountable)
8×12 strips
4°C for 6 months
Lyophilized Standard
2
4°C/-20°C
Sample/Standard Dilution Buffer
20ml
4°C
Biotin-labeled Antibody(Concentrated)
120ul
4°C (Protect from light)
Antibody Dilution Buffer
10ml
4°C
HRP-Streptavidin Conjugate(SABC)
120ul
4°C (Protect from light)
SABC Dilution Buffer
10ml
4°C
TMB Substrate
10ml
4°C (Protect from light)
Stop Solution
10ml
4°C
Wash Buffer(25X)
30ml
4°C
Plate Sealer
5
-
Other materials and equipment required:
Microplate reader with 450 nm wavelength filter
Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
CASP3: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp--Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of
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