Caspase 10 encodes a member of the cysteine-aspartic acid protease family, which plays a central role in the execution-phase of cell apoptosis. Caspase 10 cleaves and activates caspases 3 and 7, and itself is processed by caspase 8. Mutations in Caspase 10 are associated with type IIA autoimmune lymphoproliferative syndrome, non-Hodgkin lymphoma and gastric cancer. The Assay Genie Human Caspase 10 ELISA is a highly sensitive assay for the quantitative measurement of Caspase 10 in serum, blood, plasma, cell culture supernatant and tissue samples.
This immunoassay kit allows for the in vitro quantitative determination of Human CASP10 concentrations in serum plasma and other biological fluids.
Sensitivity:
0.094ng/ml
Range:
0.156-10ng/ml
Storage:
4°C for 6 months
Note:
For Research Use Only
Recovery:
Matrices listed below were spiked with certain level of Human CASP10 and the recovery rates were calculated by comparing the measured value to the expected amount of Human CASP10 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-96
92
EDTA plasma(n=5)
90-99
94
UFH plasma(n=5)
85-100
92
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human CASP10 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
93-103%
87-101%
86-104%
EDTA plasma(n=5)
83-99%
82-100%
83-94%
UFH plasma(n=5)
83-100%
80-87%
90-98%
CV(%):
Intra-Assay: CV<8% Inter-Assay: CV<10%
Component
Quantity
Storage
ELISA Microplate (Dismountable)
8×12 strips
4°C for 6 months
Lyophilized Standard
2
4°C/-20°C
Sample/Standard Dilution Buffer
20ml
4°C
Biotin-labeled Antibody(Concentrated)
120ul
4°C (Protect from light)
Antibody Dilution Buffer
10ml
4°C
HRP-Streptavidin Conjugate(SABC)
120ul
4°C (Protect from light)
SABC Dilution Buffer
10ml
4°C
TMB Substrate
10ml
4°C (Protect from light)
Stop Solution
10ml
4°C
Wash Buffer(25X)
30ml
4°C
Plate Sealer
5
-
Other materials and equipment required:
Microplate reader with 450 nm wavelength filter
Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
CASP10: Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase- 3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp--AMC and Asp-Glu-Val-Asp--AMC. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 23/17 kDa (p23/17) (depending on the splicing events) and a 12 kDa (p12) subunit. Self-associates. Interacts with FADD and CASP8. Found in a Fas signaling
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