Human C5a Recombinant Protein (RPPB2950)
- SKU:
- RPPB2950
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P01031
Description
| Product Name: | Human C5a Recombinant Protein |
| Product Code: | RPPB2950 |
| Size: | 20µg |
| Species: | Human |
| Target: | C5a |
| Synonyms: | Complement Component 5, C3 and PZP-Like Alpha-2-Macroglobulin Domain-Containing Protein 4, C5a Anaphylatoxin, Prepro-C5, CPAMD4, Anaphylatoxin C5a Analog, ECLZB, C5A, C5D, C5b, C5. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Human C5a was lyophilized from a concentrated (1mg/ml) solution in PBS, pH 7.4. |
| Solubility: | It is recommended to reconstitute the lyophilized C5a Human in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Human C5a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Human C5a should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Purity: | Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | TLQKKIEEIA AKYKHSVVKK CCYDGACVNN DETCEQRAAR ISLGPRCIKA FTECCVVASQ LRANISHKDM QLGR |
| Biological Activity: | The biological activity determined by its ability to chemoattract human monocytes using a concentration range of 1.0-10.0 ng/ml. |
Complement Component C5a (C5a) is involved in the complement system and it is encoded by the C5 gene in human. Complement C5 is cleaved into C5a and C5b. C5a has an imperative role in chemotaxis and C5b forms the 1st part of the complement membrane attack complex. Complement C5 is the 5th component of complement, which plays a central role in inflammatory and cell killing processes. C5a protein is composed of alpha and beta polypeptide chains, which are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin, which has potent spasmogenic and chemotactic activity, is derivative from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product forms a complex with the C6 complement component, and this complex is the basis for creation of the membrane attack complex, which includes supplementary complement components.
C5a Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 74 amino acids and having a molecular mass of 8.3kDa.The Human C5a is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | C5: Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. Defects in C5 are the cause of complement component 5 deficiency (C5D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705). |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 9q33-q34 Cellular Component: membrane attack complex; extracellular space; extracellular region Molecular Function:protein binding; chemokine activity; endopeptidase inhibitor activity; C5a anaphylatoxin chemotactic receptor binding; receptor binding Biological Process: in utero embryonic development; positive regulation of chemotaxis; activation of MAPK activity; cytolysis; complement activation, alternative pathway; chemotaxis; glucose homeostasis; leukocyte migration during inflammatory response; complement activation; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; cell surface receptor linked signal transduction; regulation of complement activation; response to stress; innate immune response; negative regulation of dopamine secretion; inflammatory response; complement activation, classical pathway Disease: Complement Component 5 Deficiency; Eculizumab, Poor Response To |
| NCBI Summary: | The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to a susceptibility to liver fibrosis and to rheumatoid arthritis. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P01031 |
| NCBI GenInfo Identifier: | 166900096 |
| NCBI Gene ID: | 727 |
| NCBI Accession: | P01031.4 |
| UniProt Secondary Accession: | P01031,Q14CJ0, Q27I61, |
| UniProt Related Accession: | P01031 |
| Molecular Weight: | 188,305 Da |
| NCBI Full Name: | Complement C5 |
| NCBI Synonym Full Names: | complement component 5 |
| NCBI Official Symbol: | C5�� |
| NCBI Official Synonym Symbols: | C5D; C5a; C5b; ECLZB; CPAMD4�� |
| NCBI Protein Information: | complement C5; prepro-C5; C5a anaphylatoxin; anaphylatoxin C5a analog; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4 |
| UniProt Protein Name: | Complement C5 |
| UniProt Synonym Protein Names: | C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4Cleaved into the following 4 chains:Complement C5 beta chain; Complement C5 alpha chain; C5a anaphylatoxin; Complement C5 alpha' chain |
| Protein Family: | Complement C5 |
| UniProt Gene Name: | C5�� |
| UniProt Entry Name: | CO5_HUMAN |
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