Human BTF3 Recombinant Protein (RPPB2913)

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SKU:
RPPB2913
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P20290

Description

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Product Name:Human BTF3 Recombinant Protein
Product Code:RPPB2913
Size:10µg
Species:Human
Target:BTF3
Synonyms:Transcription factor BTF3, RNA polymerase B transcription factor 3, BTF3, NACB, BTF3a; BTF3b, BETA-NAC.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:BTF3 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 50% glycerol and 2mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMRRTGAP AQADSRGRGR ARGGCPGGEA TLSQPPPRGG TRGQEPQMKE TIMNQEKLAK LQAQVRIGGK GTARRKKKVV HRTATADDKK LQFSLKKLGV NNISGIEEVN MFTNQGTVIH FNNPKVQASL AANTFTITGH AETKQLTEML PSILNQLGAD SLTSLRRLAE ALPKQSVDGK APLATGEDDD DEVPDLVENF DEASKNEAN

Basic transcription factor 3 (BTF3), which is a member of the NAC-beta family, forms a stable complex with RNA polymerase II. The BTF3 protein is vital for the initiation of transcription.

BTF3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 229 amino acids (1-206 a.a) and having a molecular mass of 24.6kDa.BTF3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:BTF3: a general transcription factor. BTF3 can form a stable complex with RNA polymerase II. An interaction partner of protein kinase CK2. Required for the initiation of transcription. Expressed at high levels in glioblastoma multiforme. Overexpressed in the microsatellite instability (MIN) phenotypes in colorectal cancer. Up-regulated strongly in the mammary gland during pregnancy, suggesting an involvement in alveolar growth. Two alternatively spliced isoforms have been described.
UniProt Protein Details:

Protein type:Transcription factor; Transcription initiation complex

Chromosomal Location of Human Ortholog: 5q13.2

Molecular Function:protein binding

Biological Process: transcription from RNA polymerase II promoter

NCBI Summary:This gene encodes the basic transcription factor 3. This protein forms a stable complex with RNA polymerase IIB and is required for transcriptional initiation. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene has multiple pseudogenes. [provided by RefSeq, Jul 2008]
UniProt Code:P20290
NCBI GenInfo Identifier:115143
NCBI Gene ID:689
NCBI Accession:P20290.1
UniProt Secondary Accession:P20290,Q13893, Q76M56, A8K510,
UniProt Related Accession:P20290
Molecular Weight:17,699 Da
NCBI Full Name:Transcription factor BTF3
NCBI Synonym Full Names:basic transcription factor 3
NCBI Official Symbol:BTF3��
NCBI Official Synonym Symbols:NACB; BTF3a; BTF3b; BETA-NAC��
NCBI Protein Information:transcription factor BTF3
UniProt Protein Name:Transcription factor BTF3
UniProt Synonym Protein Names:Nascent polypeptide-associated complex subunit beta; NAC-beta; RNA polymerase B transcription factor 3
Protein Family:Basic transcription factor
UniProt Gene Name:BTF3��
UniProt Entry Name:BTF3_HUMAN
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