Chemokines Recombinant Proteins

Human BRAK Recombinant Protein (RPPB1103)

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SKU:
RPPB1103
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O95715
Research Area:
Chemokines

Description

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Product Name:Human BRAK Recombinant Protein
Product Code:RPPB1103
Size:20µg
Species:Human
Target:BRAK
Synonyms:C-X-C motif chemokine 14, Small-inducible cytokine B14, Chemokine BRAK, Bolekine, NJAC, KS1, Kec, BMAC, MIP-2g, SCYB14, CXCL14, BRAK, MGC10687.�
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:CXCL14 was lyophilized after extensive dialysis against 20mM Tris-HCl, pH 8.5 and 1M NaCl.
Solubility:It is recommended to reconstitute the lyophilized CXCL14 in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized CXCL14 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL14 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.
Purity:Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Lys-Cys-Lys-Cys
Biological Activity:The ED50 of CXCL14 as determined by its ability to induce calcium flux of prostaglandin E2 treated THP1 human acute monocytic leukemia cells was 1.0-10.0 ng/ml.

CXCL14 is involved in immunoregulatory and inflammatory processes. BRAK protein is structurally related to the CXC (Cys-X-Cys) subfamily of cytokines. CXCL14 displays chemotactic activity for monocytes but not for lymphocytes, dendritic cells, neutrophils or macrophages. CXCL14 is involved in the homeostasis of monocyte-derived macrophages.

CXCL14 Human Recombinant produced in E.Coli is a single,�non-glycosylated, Polypeptide chain containing�77 amino acids and having a molecular mass of 9.4kDa.The CXCL14 is purified by proprietary chromatographic techniques.

UniProt Protein Function:CXCL14: Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactic for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays. Belongs to the intercrine alpha (chemokine CxC) family.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Secreted; Chemokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: Golgi apparatus; extracellular space

Molecular Function:chemokine activity

Biological Process: cell-cell signaling; immune response; negative regulation of myoblast differentiation; chemotaxis; signal transduction; inner ear development

NCBI Summary:This antimicrobial gene belongs to the cytokine gene family which encode secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC (Cys-X-Cys) subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes but not for lymphocytes, dendritic cells, neutrophils or macrophages. It has been implicated that this cytokine is involved in the homeostasis of monocyte-derived macrophages rather than in inflammation. [provided by RefSeq, Sep 2014]
UniProt Code:O95715
NCBI GenInfo Identifier:292495003
NCBI Gene ID:9547
NCBI Accession:O95715.2
UniProt Secondary Accession:O95715,Q6UW97, Q86U69, Q9BTR1, Q9NS21, B3KQU8,
UniProt Related Accession:O95715
Molecular Weight:13,078 Da
NCBI Full Name:C-X-C motif chemokine 14
NCBI Synonym Full Names:chemokine (C-X-C motif) ligand 14
NCBI Official Symbol:CXCL14��
NCBI Official Synonym Symbols:KEC; KS1; BMAC; BRAK; NJAC; MIP2G; MIP-2g; SCYB14��
NCBI Protein Information:C-X-C motif chemokine 14; bolekine; MIP-2 gamma; chemokine BRAK; breast and kidney; tumor-suppressing chemokine; small-inducible cytokine B14; CXC chemokine in breast and kidney; small inducible cytokine subfamily B (Cys-X-Cys), member 14 (BRAK)
UniProt Protein Name:C-X-C motif chemokine 14
UniProt Synonym Protein Names:Chemokine BRAK; MIP-2G; Small-inducible cytokine B14
UniProt Gene Name:CXCL14��
UniProt Entry Name:CXL14_HUMAN
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