Enzymes Recombinant Proteins

Human BPHL Recombinant Protein (RPPB1452)

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RPPB1452

Description

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Product Name:Human BPHL Recombinant Protein
Product Code:RPPB1452
Size:20µg
Species:Human
Target:BPHL
Synonyms:Biphenyl Hydrolase-Like (serine hydrolase), Bph-rp, Breast Epithelial Mucin-Associated Antigen, MCNAA, VACVASE, MGC41865, MGC125930.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:The BPHL protein solution (0.5mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0)1mM DTT, 50mM NaCl and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.�Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MSVTSAKVAV NGVQLHYQQT GEGDHAVLLL PGMLGSGETD FGPQLKNLNK KLFTVVAWDP RGYGHSRPPD RDFPADFFER DAKDAVDLMK ALKFKKVSLL GWSDGGITAL IAAAKYPSYI HKMVIWGANA YVTDEDSMIY EGIRDVSKWS ERTRKPLEAL YGYDYFARTC EKWVDGIRQF KHLPDGNICR HLLPRVQCPA LIVHGEKDPL VPRFHADFIH KHVKGSRLHL MPEGKHNLHL RFADEFNKLA EDFLQ

BPHL is a Serine hydrolase that is a part of the AB hydrolase superfamily which catalyzes the hydrolytic activation of amino acid ester prodrugs of nucleoside analogs. BPHL is expressed large quantities in liver and kidney and in minor quantities in heart, intestine and skeletal muscle. BPHL takes part in detoxification processes and is a specific alpha-amino acid ester hydrolase which favors small, hydrophobic, and aromatic side chains and does not have a strict necessity for the leaving group except for favoring a primary alcohol.

BPHL produced in E.Coli is a single, non-glycosylated polypeptide chain containing 275 amino acids (38-291a.a.) and having a molecular mass of 31.1kDa.BPHL is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:BPHL: Serine hydrolase that catalyzes the hydrolytic activation of amino acid ester prodrugs of nucleoside analogs such as valacyclovir and valganciclovir. Activates valacyclovir to acyclovir. May play a role in detoxification processes. It is a specific alpha-amino acid ester hydrolase that prefers small, hydrophobic, and aromatic side chains and does not have a stringent requirement for the leaving group other than preferring a primary alcohol. Belongs to the AB hydrolase superfamily. Lipase family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: EC 3.1.-.-; HydrolaseChromosomal Location of Human Ortholog: 6p25Cellular Component: mitochondrial outer membrane; mitochondrionMolecular Function: alpha-amino-acid esterase activity; hydrolase activityBiological Process: amino acid metabolic process; response to toxin; xenobiotic metabolic process
UniProt Protein Details:
NCBI Summary:This gene encodes a member of the serine protease family of hydrolytic enzymes which contain a serine in their active site. The encoded protein may play a role in activation of the antiviral prodrug valacyclovir. Alternatively spliced transcript variants have been described.[provided by RefSeq, Jan 2009]
UniProt Code:Q86WA6
NCBI GenInfo Identifier:39931107
NCBI Gene ID:670
NCBI Accession:Q86WA6.1
UniProt Secondary Accession:Q86WA6,Q00306, Q13855, Q3KP51
UniProt Related Accession:Q86WA6
Molecular Weight:31,107 Da
NCBI Full Name:Valacyclovir hydrolase
NCBI Synonym Full Names:biphenyl hydrolase like
NCBI Official Symbol:BPHL��
NCBI Official Synonym Symbols:MCNAA; BPH-RP; VACVASE��
NCBI Protein Information:valacyclovir hydrolase
UniProt Protein Name:Valacyclovir hydrolase
UniProt Synonym Protein Names:Biphenyl hydrolase-like protein; Biphenyl hydrolase-related protein; Bph-rp; Breast epithelial mucin-associated antigen; MCNAA
Protein Family:Valacyclovir hydrolase
UniProt Gene Name:BPHL��
UniProt Entry Name:BPHL_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q86WA6
Research Area:
Enzymes
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