Enzymes Recombinant Proteins

Human BLMH Recombinant Protein (RPPB1446)

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SKU:
RPPB1446
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q13867
Research Area:
Enzymes

Description

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Product Name:Human BLMH Recombinant Protein
Product Code:RPPB1446
Size:20µg
Species:Human
Target:BLMH
Synonyms:BMH, BH, BLM hydrolase.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:The BLMH protein solution (1mg/1ml) is formulated in 20mM Tris-HCl Buffer (pH 8.0) and 10% Glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.�Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MSSSGLNSEK VAALIQKLNS DPQFVLAQNV GTTHDLLDIC LKRATVQRAQ HVFQHAVPQE GKPITNQKSS GRCWIFSCLN VMRLPFMKKL NIEEFEFSQS YLFFWDKVER CYFFLSAFVD TAQRKEPEDG RLVQFLLMNP ANDGGQWDML VNIVEKYGVI PKKCFPESYT TEATRRMNDI LNHKMREFCI RLRNLVHSGA TKGEISATQD VMMEEIFRVV CICLGNPPET FTWEYRDKDK NYQKIGPITP LEFYREHVKP LFNMEDKICL VNDPRPQHKY NKLYTVEYLS NMVGGRKTLY NNQPIDFLKK MVAASIKDGE AVWFGCDVGK HFNSKLGLSD MNLYDHELVF GVSLKNMNKA ERLTFGESLM THAMTFTAVS EKDDQDGAFT KWRVENSWGE DHGHKGYLCM TDEWFSEYVY EVVVDRKHVP EEVLAVLEQE PIILPAWDPM GALAE
Biological Activity:Specific activity: > 1,000 pmole/min/ug. Measured by the hydrolysis of Met-AMC at pH 7.5, at 37C.

BLMH is affiliate to the papain superfamily of the cysteine protease and the peptidase C1 family. BLMH is a cytoplasmic cysteinepeptidase usually found as a homohexamer. The standard physiological role of BLMH has not been determined, but it shields normal and malignant cells from the glycopeptide antitumor drug BLM. BLMH catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxyamide bond of its B-aminoalaninamide moiety and in addition demonstrates general aminopeptidase activity.

BLMH produced in E.Coli is a single, non-glycosylated polypeptide chain containing 475 amino acids (1-455a.a.) and having a molecular mass of 54.7kDa.BLMH is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:BLMH: The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B- aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity. Belongs to the peptidase C1 family.
UniProt Protein Details:

Protein type:EC 3.4.22.40; Protease

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function:aminopeptidase activity; carboxypeptidase activity; cysteine-type peptidase activity; protein binding

Biological Process: protein polyubiquitination

Disease: Alzheimer Disease

NCBI Summary:Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily. [provided by RefSeq, Jul 2008]
UniProt Code:Q13867
NCBI GenInfo Identifier:3023394
NCBI Gene ID:642
NCBI Accession:Q13867.1
UniProt Secondary Accession:Q13867,Q53F86, Q9UER9, B2R796,
UniProt Related Accession:Q13867
Molecular Weight:52,562 Da
NCBI Full Name:Bleomycin hydrolase
NCBI Synonym Full Names:bleomycin hydrolase
NCBI Official Symbol:BLMH��
NCBI Official Synonym Symbols:BH; BMH��
NCBI Protein Information:bleomycin hydrolase
UniProt Protein Name:Bleomycin hydrolase
Protein Family:Bleomycin hydrolase
UniProt Gene Name:BLMH��
UniProt Entry Name:BLMH_HUMAN
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