Human BGN Recombinant Protein (RPPB2887)
- SKU:
- RPPB2887
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P21810
Description
| Product Name: | Human BGN Recombinant Protein |
| Product Code: | RPPB2887 |
| Size: | 10µg |
| Species: | Human |
| Target: | BGN |
| Synonyms: | BGN, DSPG1, MRLS, PG-S1, PGI, SEMDX, SLRR1A, Biglycan, Bone/cartilage proteoglycan I, Biglycan Proteoglycan, MRLS. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The BGN solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | ADPDEEASGA DTSGVLDPDS VTPTYSAMCP FGCHCHLRVV QCSDLGLKSV PKEISPDTTL LDLQNNDISE LRKDDFKGLQ HLYALVLVNN KISKIHEKAF SPLRKLQKLY ISKNHLVEIP PNLPSSLVEL RIHDNRIRKV PKGVFSGLRN MNCIEMGGNP LENSGFEPGA FDGLKLNYLR ISEAKLTGIP KDLPETLNEL HLDHNKIQAI ELEDLLRYSK LYRLGLGHNQ IRMIENGSLS FLPTLRELHL DNNKLARVPS GLPDLKLLQV VYLHSNNITK VGVNDFCPMG FGVKRAYYNG ISLFNNPVPY WEVQPATFRC VTDRLAIQFG NYKKHHHHHH |
| Biological Activity: | The ED50 for this effect is ? 20 ug/ml. The specific activity is measured by inhibiting the cell growth using 3T3? L1 mouse embryonic �fibroblast �adipose-like cells. |
Biglycan (BGN) is a small cellular or pericellular matrix proteoglycan which takes part in assembly of collagen fibrils and muscle regeneration. BGN is closely correlated in structure to two other small proteoglycans, decorin and fibromodulin. BGN interacts with several proteins involved in muscular dystrophy, including alpha-dystroglycan, alpha- and gamma-sarcoglycan and collagen VI. BGN is also critical for the assembly of the dystrophin-associated protein complex.
BGN produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 340 amino acids (38-368 a.a.) and having a molecular mass of 38.3kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).BGN is expressed with a 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | BGN: May be involved in collagen fiber assembly. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: Xq28 Cellular Component: extracellular matrix; proteinaceous extracellular matrix; lysosomal lumen; transport vesicle; cell surface; Golgi lumen; extracellular region; sarcolemma Molecular Function:glycosaminoglycan binding; extracellular matrix binding; extracellular matrix structural constituent Biological Process: chondroitin sulfate metabolic process; extracellular matrix organization and biogenesis; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; chondroitin sulfate catabolic process; pathogenesis; blood vessel remodeling; peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan; dermatan sulfate biosynthetic process |
| NCBI Summary: | The protein encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to two other small proteoglycans, decorin and fibromodulin. The encoded protein and decorin are thought to be the result of a gene duplication. Decorin contains one attached glycosaminoglycan chain, while this protein probably contains two chains. For this reason, this protein is called biglycan. This protein plays a role in assembly of collagen fibrils and muscle regeneration. It interacts with several proteins involved in muscular dystrophy, including alpha-dystroglycan, alpha- and gamma-sarcoglycan and collagen VI, and it is critical for the assembly of the dystrophin-associated protein complex. [provided by RefSeq, Nov 2009] |
| UniProt Code: | P21810 |
| NCBI GenInfo Identifier: | 266762 |
| NCBI Gene ID: | 633 |
| NCBI Accession: | P21810.2 |
| UniProt Secondary Accession: | P21810,P13247, D3DWU3, |
| UniProt Related Accession: | P21810 |
| Molecular Weight: | 368 |
| NCBI Full Name: | Biglycan |
| NCBI Synonym Full Names: | biglycan |
| NCBI Official Symbol: | BGN�� |
| NCBI Official Synonym Symbols: | PGI; DSPG1; PG-S1; SLRR1A�� |
| NCBI Protein Information: | biglycan; biglycan proteoglycan; bone/cartilage proteoglycan I; bone/cartilage proteoglycan-I; small leucine-rich protein 1A; dermatan sulphate proteoglycan I |
| UniProt Protein Name: | Biglycan |
| UniProt Synonym Protein Names: | Bone/cartilage proteoglycan I; PG-S1 |
| Protein Family: | Biglycan |
| UniProt Gene Name: | BGN�� |
| UniProt Entry Name: | PGS1_HUMAN |
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