Cytokines Recombinant Proteins

Human BD 1 Recombinant Protein (RPPB0068)

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RPPB0068

Description

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Product Name:Human BD 1 Recombinant Protein
Product Code:RPPB0068
Size:20µg
Species:Human
Target:BD 1
Synonyms:Beta-defensin 1, BD-1, Defensin beta 1, hBD-1, HBD1, HBP1, DEFB1, HBD-1, HBP-1, DEFB101, DEFB-1, MGC51822.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:The Human BD-1 was lyophilized from a concentrated (1mg/ml) solution containing 20mM PBS pH-7.4 and 130mM sodium chloride.
Solubility:It is recommended to reconstitute the lyophilized Beta Defensin-1 in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized Beta Defensin-1 Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BD-1 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.
Purity:Greater than 98.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:GNFLTGLGHR SDHYNCVSSG GQCLYSACPI FTKIQGTCYR GKAKCCK
Biological Activity:Determined by its ability to chemoattract CD34+ dendritic cells using a concentration range of 100-1000ng/ml corresponding to a specific activity of 1,000-10,000IU/mg.

The Defensin family are highly similar in their protein sequence and are microbicidal & cytotoxic peptides made by neutrophils. Beta Defensin-1 is an antimicrobial peptide having the resistance of epithelial surfaces to microbial colonization. Beta Defensin-1 has close proximity to Defensin Alpha-1 and has been implicated in the pathogenesis of cystic fibrosis.Skin of patients having atopic dermatitis patients and mycosis fungoides (non-lesional and lesional) show lower human Beta Defensin-1 mRNA expression and higher human Beta Defensin-2 and human Beta Defensin-3 mRNA expression. Beta Defensin is highly expressed by epithelial cells. Beta-defensin 1 may play a role in the pathogenesis of severe sepsis. Variation in human Beta Defensin-1 contributes to asthma diagnosis, with apparent gender-specific effects. Human Beta Defensin-3 is a dimer, while Human BD-1 and Human BD-2 are monomeric. The expression of Human BD1 is correlated with induction profiles in gingival keratinocytes. The level of expression of human DEFB1 mRNA is lower than that of human BD3 and human BD-2 in reconstructed epidermis.Human BD1 is down-regulated in human prostatic and renal carcinomas.

Beta Defensin-1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 47 amino acids and having a molecular mass of 5 kDa.The BD-1 is purified by proprietary chromatographic techniques.

UniProt Protein Function:defensin, beta 1: Has bactericidal activity. Belongs to the beta-defensin family.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: extracellular space; Golgi lumen; extracellular region

Biological Process: G-protein coupled receptor protein signaling pathway; defense response to Gram-positive bacterium; innate immune response in mucosa; response to bacterium; antibacterial humoral response; innate immune response; immune response; response to testosterone stimulus; chemotaxis; acute inflammatory response

NCBI Summary:Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in the resistance of epithelial surfaces to microbial colonization. This gene maps in close proximity to defensin family member, defensin, alpha 1 and has been implicated in the pathogenesis of cystic fibrosis. [provided by RefSeq, Jul 2008]
UniProt Code:P60022
NCBI GenInfo Identifier:38503374
NCBI Gene ID:1672
NCBI Accession:P60022.1
UniProt Secondary Accession:P60022,Q09753,
UniProt Related Accession:P60022
Molecular Weight:7,420 Da
NCBI Full Name:Beta-defensin 1
NCBI Synonym Full Names:defensin, beta 1
NCBI Official Symbol:DEFB1��
NCBI Official Synonym Symbols:BD1; HBD1; DEFB-1; DEFB101��
NCBI Protein Information:beta-defensin 1; BD-1; beta-defensin-1
UniProt Protein Name:Beta-defensin 1
UniProt Synonym Protein Names:Defensin, beta 1
UniProt Gene Name:DEFB1��
UniProt Entry Name:DEFB1_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P60022
Research Area:
Cytokines
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