Human BCDIN3D Recombinant Protein (RPPB2860)

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SKU:
RPPB2860
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q7Z5W3

Description

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Product Name:Human BCDIN3D Recombinant Protein
Product Code:RPPB2860
Size:10µg
Species:Human
Target:BCDIN3D
Synonyms:Pre-miRNA 5'-monophosphate methyltransferase, BCDIN3 domain-containing protein, BCDIN3D.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:BCDIN3D protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 40% glycerol, 1mM DTT and 2mM EDTA.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMAVPTEL DGGSVKETAA EEESRVLAPG AAPFGNFPHY SRFHPPEQRL RLLPPELLRQ LFPESPENGP ILGLDVGCNS GDLSVALYKH FLSLPDGETC SDASREFRLL CCDIDPVLVK RAEKECPFPD ALTFITLDFM NQRTRKVLLS SFLSQFGRSV FDIGFCMSIT MWIHLNHGDH GLWEFLAHLS SLCHYLLVEP QPWKCYRAAA RRLRKLGLHD FDHFHSLAIR GDMPNQIVQI LTQDHGMELI CCFGNTSWDR SLLLFRAKQT IETHPIPESL IEKGKEKNRL SFQKQ

BCDIN3D is a member of the methyltransferase superfamily and contains 1 Bin3-type SAM domain. BCDIN3D acts in the catalysis of the transfer of a methyl group to an acceptor molecule. BCDIN3D is an O-methyltransferase which specifically dimethylates the 5' monophosphate of pre-miRNAs, serving as a negative regulator of miRNA processing. BCDIN3D mediates the methylation of pre-miR-145, as well as other pre-miRNAs.

BCDIN3D Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 315 amino acids (1-292 a.a) and having a molecular mass of 35kDa.BCDIN3D is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:BCDIN3D: Probable methyltransferase. Belongs to the methyltransferase superfamily.
UniProt Protein Details:

Protein type:EC 2.1.1.-; Methyltransferase

Chromosomal Location of Human Ortholog: 12q13.12

Cellular Component: cytoplasm; nucleus

Molecular Function:O-methyltransferase activity; protein binding; RNA methyltransferase activity

Biological Process: RNA methylation

UniProt Code:Q7Z5W3
NCBI GenInfo Identifier:74738762
NCBI Gene ID:144233
NCBI Accession:Q7Z5W3.1
UniProt Secondary Accession:Q7Z5W3,A8K829,
UniProt Related Accession:Q7Z5W3
Molecular Weight:33,200 Da
NCBI Full Name:Pre-miRNA 5'-monophosphate methyltransferase
NCBI Synonym Full Names:BCDIN3 domain containing RNA methyltransferase
NCBI Official Symbol:BCDIN3D��
NCBI Protein Information:pre-miRNA 5'-monophosphate methyltransferase
UniProt Protein Name:Pre-miRNA 5'-monophosphate methyltransferase
UniProt Synonym Protein Names:BCDIN3 domain-containing protein
Protein Family:Pre-miRNA 5'-monophosphate methyltransferase
UniProt Gene Name:BCDIN3D��
UniProt Entry Name:BN3D2_HUMAN
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