Description
| Product Name: | Human BCAT2 Recombinant Protein |
| Product Code: | RPPB1438 |
| Size: | 10µg |
| Species: | Human |
| Target: | BCAT2 |
| Synonyms: | Branched-chain-amino-acid aminotransferase mitochondrial, BCAT(m), Placental protein 18, PP18, BCAT2, BCATM, BCT2, ECA40, BCAM. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The BCAT2 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 7.5), 10% glycerol, 0.2M NaCl and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMASSSF KAADLQLEMT QKPHKKPGPG EPLVFGKTFT DHMLMVEWND KGWGQPRIQP FQNLTLHPAS SSLHYSLQLF EGMKAFKGKD QQVRLFRPWL NMDRMLRSAM RLCLPSFDKL ELLECIRRLI EVDKDWVPDA AGTSLYVRPV LIGNEPSLGV SQPTRALLFV ILCPVGAYFP GGSVTPVSLL ADPAFIRAWV GGVGNYKLGG NYGPTVLVQQ EALKRGCEQV LWLYGPDHQL TEVGTMNIFV YWTHEDGVLE LVTPPLNGVI LPGVVRQSLL DMAQTWGEFR VVERTITMKQ LLRALEEGRV REVFGSGTAC QVCPVHRILY KDRNLHIPTM ENGPELILRF QKELKEIQYG IRAHEWMFPV |
Branched Chain Amino-Acid Transaminase 2 (BCAT2) is a member of the class-IV pyridoxal-phosphate-dependent aminotransferase family. BCAT2 is a branched chain aminotransferase found in mitochondria. BCAT2 forms a dimer which catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. In addition, BCAT2 may actn as a transporter of branched chain alpha-keto acids.
BCAT2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 390 amino acids (28-392) and having a molecular mass of 43.9kDa.BCAT2 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | BCAT2: Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids. Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. Homodimer. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - valine, leucine and isoleucine biosynthesis; EC 2.6.1.42; Mitochondrial; Transferase; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis Chromosomal Location of Human Ortholog: 19q13 Cellular Component: mitochondrion; mitochondrial matrix Molecular Function:branched-chain-amino-acid transaminase activity Biological Process: valine metabolic process; branched chain family amino acid catabolic process; leucine metabolic process; branched chain family amino acid biosynthetic process; isoleucine catabolic process |
| NCBI Summary: | This gene encodes a branched chain aminotransferase found in mitochondria. The encoded protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009] |
| UniProt Code: | O15382 |
| NCBI GenInfo Identifier: | 20455470 |
| NCBI Gene ID: | 587 |
| NCBI Accession: | O15382.2 |
| UniProt Secondary Accession: | O15382,O00269, Q96KG1, Q9BTB6, Q9BUU6, B2RB87, |
| UniProt Related Accession: | O15382 |
| Molecular Weight: | 33,777 Da |
| NCBI Full Name: | Branched-chain-amino-acid aminotransferase, mitochondrial |
| NCBI Synonym Full Names: | branched chain amino-acid transaminase 2, mitochondrial |
| NCBI Official Symbol: | BCAT2�� |
| NCBI Official Synonym Symbols: | BCAM; BCT2; PP18; BCATM�� |
| NCBI Protein Information: | branched-chain-amino-acid aminotransferase, mitochondrial; placental protein 18; branched chain aminotransferase 2, mitochondrial |
| UniProt Protein Name: | Branched-chain-amino-acid aminotransferase, mitochondrial |
| UniProt Synonym Protein Names: | Placental protein 18; PP18 |
| UniProt Gene Name: | BCAT2�� |
| UniProt Entry Name: | BCAT2_HUMAN |
