Human Atherosclerosis Array (20 targets) (SARB0024)

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SKU:
SARB0024
Product Type:
Protein Array
Analytes:
Eotaxin-1 (CCL11)
Analytes:
GCSF
Analytes:
GDNF
Analytes:
GM-CSF
Analytes:
ICAM-1 (CD54)
Analytes:
IFN-gamma
Analytes:
IL-1 alpha (IL-1 F1)
Analytes:
IL-1 beta (IL-1 F2)
Analytes:
IL-1 R1
Analytes:
IL-1 R2
Analytes:
MCP-1 (CCL2)
Analytes:
M-CSF
Analytes:
TGF beta 1
Analytes:
TGF beta 2
Analytes:
TGF beta 3
Analytes:
TNF alpha
Analytes:
TNF beta (TNFSF1B)
Analytes:
MIP-3 alpha (CCL20)
Analytes:
PDGF-BB
Analytes:
RANTES (CCL5)
Reactivity:
Human
Applications:
Multiplex Array

Description

system_update_altDatasheet
Product Sku:SARB0024
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:20
Gene Symbols:CCL11, CCL2, CCL20, CCL5, CSF1, CSF2, CSF3, GDNF, ICAM1, IFNG|IL1A, IL1B, IL1R1, IL1R2, LTA, PDGFB, TGFB1, TGFB2, TGFB3, TNF
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:MAPK Signaling / NFkB Signaling
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
Eotaxin-1 (CCL11)
  • Gene Symbol: CCL11
  • Gene ID: 6356
  • Accession #: P51671
GCSF
  • Gene Symbol: CSF3
  • Gene ID: 1440
  • Accession #: P09919
GDNF
  • Gene Symbol: GDNF
  • Gene ID: 2668
  • Accession #: P39905
GM-CSF
  • Gene Symbol: CSF2
  • Gene ID: 1437
  • Accession #: P04141
ICAM-1 (CD54)
  • Gene Symbol: ICAM1
  • Gene ID: 3383
  • Accession #: P05362
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: 3458
  • Accession #: P01579
IL-1 alpha (IL-1 F1)
  • Gene Symbol: IL1A
  • Gene ID: 3552
  • Accession #: P01583
IL-1 beta (IL-1 F2)
  • Gene Symbol: IL1B
  • Gene ID: 3553
  • Accession #: P01584
IL-1 R1
  • Gene Symbol: IL1R1
  • Gene ID: 3554
  • Accession #: P14778
IL-1 R2
  • Gene Symbol: IL1R2
  • Gene ID: 7850
  • Accession #: P27930
MCP-1 (CCL2)
  • Gene Symbol: CCL2
  • Gene ID: 6347
  • Accession #: P13500
M-CSF
  • Gene Symbol: CSF1
  • Gene ID: 1435
  • Accession #: P09603
TGF beta 1
  • Gene Symbol: TGFB1
  • Gene ID: 7040
  • Accession #: P01137
TGF beta 2
  • Gene Symbol: TGFB2
  • Gene ID: 7042
  • Accession #: P61812
TGF beta 3
  • Gene Symbol: TGFB3
  • Gene ID: 7043
  • Accession #: P10600
TNF alpha
  • Gene Symbol: TNF
  • Gene ID: 7124
  • Accession #: P01375
TNF beta (TNFSF1B)
  • Gene Symbol: LTA
  • Gene ID: 4049
  • Accession #: P01374
MIP-3 alpha (CCL20)
  • Gene Symbol: CCL20
  • Gene ID: 6364
  • Accession #: P78556
PDGF-BB
  • Gene Symbol: PDGFB
  • Gene ID: 5155
  • Accession #: P01127
RANTES (CCL5)
  • Gene Symbol: CCL5
  • Gene ID: 6352
  • Accession #: P13501
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human Atherosclerosis Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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