Human ATG3 Recombinant Protein (RPPB2812)

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SKU:
RPPB2812
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9NT62

Description

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Product Name:Human ATG3 Recombinant Protein
Product Code:RPPB2812
Size:20µg
Species:Human
Target:ATG3
Synonyms:APG3, APG3-LIKE, APG3L, PC3-96, Ubiquitin-like-conjugating enzyme ATG3, Autophagy-related protein 3, hApg3, PC3-96, ATG3.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:ATG3 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMQNVINT VKGKALEVAE YLTPVLKESK FKETGVITPE EFVAAGDHLV HHCPTWQWAT GEELKVKAYL PTGKQFLVTK NVPCYKRCKQ MEYSDELEAI IEEDDGDGGW VDTYHNTGIT GITEAVKEIT LENKDNIRLQ DCSALCEEEE DEDEGEAADM EEYEESGLLE TDEATLDTRK IVEACKAKTD AGGEDAILQT RTYDLYITYD KYYQTPRLWL FGYDEQRQPL TVEHMYEDIS QDHVKKTVTI ENHPHLPPPP MCSVHPCRHA EVMKKIIETV AEGGGELGVH MYLLIFLKFV QAVIPTIEYD YTRHFTM

Autophagy Related 3 (ATG3) is a conjugating enzyme essential for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. ATG3 is localizes to the cytoplasm and is expressed in tissues with predominant levels found in kidney, placenta, liver, heart and skeletal muscle. ATG3 catalyzes a reaction which is necessary for autophagy. That reaction is the formation of the Atg8-phosphatidylethanolamine (ATG-PE) conjugate.

ATG3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 337 amino acids (1-314 a.a) and having a molecular mass of 38.3kDa. ATG3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:ATG3: an E2-like enzyme essential for Apg8p lipidation.
UniProt Protein Details:

Protein type:Ubiquitin conjugating system; Enzyme, cellular metabolism; Autophagy; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 3q13.2

Cellular Component: cytoplasmic ubiquitin ligase complex; cytosol

Molecular Function:APG12 conjugating enzyme activity; APG8 conjugating enzyme activity; enzyme binding; protein binding; small conjugating protein ligase activity

Biological Process: autophagic vacuole formation; macroautophagy; mitochondrial fragmentation during apoptosis; mitochondrion degradation; protein modification process; protein targeting to membrane; protein ubiquitination

NCBI Summary:This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]
UniProt Code:Q9NT62
NCBI GenInfo Identifier:61212142
NCBI Gene ID:64422
NCBI Accession:Q9NT62.1
UniProt Secondary Accession:Q9NT62,Q6PKC5, Q9H6L9,
UniProt Related Accession:Q9NT62
Molecular Weight:35,467 Da
NCBI Full Name:Ubiquitin-like-conjugating enzyme ATG3
NCBI Synonym Full Names:autophagy related 3
NCBI Official Symbol:ATG3��
NCBI Official Synonym Symbols:APG3; APG3L; PC3-96; APG3-LIKE��
NCBI Protein Information:ubiquitin-like-conjugating enzyme ATG3
UniProt Protein Name:Ubiquitin-like-conjugating enzyme ATG3
UniProt Synonym Protein Names:Autophagy-related protein 3; APG3-like; hApg3; Protein PC3-96
Protein Family:Autophagy-related protein
UniProt Gene Name:ATG3��
UniProt Entry Name:ATG3_HUMAN
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