Human ATG10 Recombinant Protein (RPPB2811)

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SKU:
RPPB2811
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9H0Y0

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human ATG10 Recombinant Protein
Product Code:RPPB2811
Size:20µg
Species:Human
Target:ATG10
Synonyms:Autophagy Related Protein 10, ATG10 Autophagy Related 10 Homolog (S. Cerevisiae), Ubiquitin-Like-Conjugating Enzyme ATG10, APG10 Autophagy 10-Like (S. Cerevisiae), APG10-Like, APG10L, Pp12616, DKFZP586I0418, FLJ13954, EC 6.3.2.-.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The ATG10 solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 1mM DTT and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMEEDEFI GEKTFQRYCA EFIKHSQQIG DSWEWRPSKD CSDGYMCKIH FQIKNGSVMS HLGASTHGQT CLPMEEAFEL PLDDCEVIET AAASEVIKYE YHVLYSCSYQ VPVLYFRASF LDGRPLTLKD IWEGVHECYK MRLLQGPWDT ITQQEHPILG QPFFVLHPCK TNEFMTPVLK NSQKINKNVN YITSWLSIVG PVVGLNLPLS YAKATSQDER NVP

Ubiquitin-like-conjugating enzyme ATG10 (ATG10) is a 220 amino acid protein which localizes to the cytoplasm and has a role in autophagy, specifically acting as an E2-like enzyme providing Atg recognition sites during autophagosome synthesis. ATG10 functions as an E2-like enzyme which catalyzes the conjugation of ATG12 to ATG5, which is required for autophagy. In addition, ATG10 interacts with ATG12 in human embryonic kidney cells in the presence of ATG7. ATG10 probably serves as an ATG5-recognition molecule. Furthermore, ATG10 has a role in adenovirus-mediated cell lysis.

ATG10 Human Recombinant produced in E. coli is a single polypeptide chain containing 243 amino acids (1-220) and having a molecular mass of 27.7 kDa.ATG10 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:ATG10: E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3. Belongs to the ATG10 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 6.3.2.-; Autophagy; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 5q14.1

Cellular Component: cytosol; intracellular

Molecular Function:APG12 conjugating enzyme activity

Biological Process: autophagy; macroautophagy; positive regulation of protein modification process; protein amino acid lipidation; protein modification by small protein conjugation

NCBI Summary:Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM, Mar 2008]
UniProt Code:Q9H0Y0
NCBI GenInfo Identifier:62286633
NCBI Gene ID:83734
NCBI Accession:Q9H0Y0.1
UniProt Secondary Accession:Q9H0Y0,Q6PIX1, Q9H842, B2RE09,
UniProt Related Accession:Q9H0Y0
Molecular Weight:14,217 Da
NCBI Full Name:Ubiquitin-like-conjugating enzyme ATG10
NCBI Synonym Full Names:autophagy related 10
NCBI Official Symbol:ATG10��
NCBI Official Synonym Symbols:APG10; APG10L; pp12616��
NCBI Protein Information:ubiquitin-like-conjugating enzyme ATG10
UniProt Protein Name:Ubiquitin-like-conjugating enzyme ATG10
UniProt Synonym Protein Names:Autophagy-related protein 10; APG10-like
Protein Family:Ubiquitin-like-conjugating enzyme
UniProt Gene Name:ATG10��
UniProt Entry Name:ATG10_HUMAN
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system_update_altDatasheetHuman ATG10 Recombinant Protein Ubiquitin-Like-Conjugating Enzyme ATG10 (ATG10) is a ubiquitous 28kDa member of the ATG10 family protein. ATG10 acts as an E2-like enzyme