Enzymes Recombinant Proteins

Human ASMT Recombinant Protein (RPPB1418)

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SKU:
RPPB1418
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P46597
Research Area:
Enzymes

Description

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Product Name:Human ASMT Recombinant Protein
Product Code:RPPB1418
Size:20µg
Species:Human
Target:ASMT
Synonyms:HIOMT, HIOMTY, Acetylserotonin O-methyltransferase , Hydroxyindole O-methyltransferase , ASMT.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:The ASMT solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0) , 1M Urea and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSSEDQAYR LLNDYANGFM VSQVLFAACE LGVFDLLAEA PGPLDVAAVA AGVRASAHGT ELLLDICVSL KLLKVETRGG KAFYRNTELS SDYLTTVSPT SQCSMLKYMG RTSYRCWGHL ADAVREGRNQ YLETFGVPAE ELFTAIYRSE GERLQFMQAL QEVWSVNGRS VLTAFDLSVF PLMCDLGGDF FKDPLPEADL YILARVLHDW ADGKCSHLLE RIYHTCKPGG GILVIESLLD EDRRGPLLTQ LYSLNMLVQT EGQERTPTHY HMLLSSAGFR DFQFKKTGAI YDAILARK

ASMT is a member of the methyltransferase superfamily. ASMT participates in melatonin biosynthesis. ASMT Expressed in brain, retina and pineal gland, ASMT utilities to catalyze the final reaction in the synthesis of melatonin, particularly the conversion of S-adenosyl-L-methionine and N-acetylserotonin to S-adenosyl-Lhomocysteine and melatonin.

ASMT Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 318 amino acids (1-298 a.a) and having a molecular mass of 35.3kDa.ASMT is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:ASMT: Produces melatonin (N-acetyl-5-methoxytryptamine) from N-acetylserotonin. Belongs to the methyltransferase superfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Amino Acid Metabolism - tryptophan; EC 2.1.1.4; Methyltransferase

Chromosomal Location of Human Ortholog: Xp22.33 and Yp11.2

Cellular Component: cytosol

Molecular Function:acetylserotonin O-methyltransferase activity; identical protein binding; O-methyltransferase activity; protein homodimerization activity; S-methyltransferase activity

Biological Process: indolalkylamine biosynthetic process; melatonin biosynthetic process; translation

NCBI Summary:This gene belongs to the methyltransferase superfamily, and is located in the pseudoautosomal region (PAR) at the end of the short arms of the X and Y chromosomes. The encoded enzyme catalyzes the final reaction in the synthesis of melatonin, and is abundant in the pineal gland. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Jan 2010]
UniProt Code:P46597
NCBI GenInfo Identifier:1170276
NCBI Gene ID:438
NCBI Accession:P46597.1
UniProt Secondary Accession:P46597,Q16598, Q5JQ72, Q5JQ73, B2RC33,
UniProt Related Accession:P46597
Molecular Weight:Calculated MW: 33kDa/38kDa/41kDaObserved MW: 52kDa
NCBI Full Name:Acetylserotonin O-methyltransferase
NCBI Synonym Full Names:acetylserotonin O-methyltransferase
NCBI Official Symbol:ASMT��
NCBI Official Synonym Symbols:ASMTY; HIOMT; HIOMTY��
NCBI Protein Information:acetylserotonin O-methyltransferase
UniProt Protein Name:Acetylserotonin O-methyltransferase
UniProt Synonym Protein Names:Hydroxyindole O-methyltransferase; HIOMT
Protein Family:Acetylserotonin O-methyltransferase
UniProt Gene Name:ASMT��
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