Human ASF1B Recombinant Protein (RPPB2806)

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SKU:
RPPB2806
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9NVP2

Description

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Product Name:Human ASF1B Recombinant Protein
Product Code:RPPB2806
Size:20µg
Species:Human
Target:ASF1B
Synonyms:Histone chaperone ASF1B, Anti-silencing function protein 1 homolog B, hAsf1, hAsf1b, CCG1-interacting factor A-II, CIA-II, hCIA-II, ASF1B.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:ASF1B protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.15M NaCl and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85% as determined by SDS-PAGE.
Amino Acid Sequence:MAKVSVLNVA VLENPSPFHS PFRFEISFEC SEALADDLEW KIIYVGSAES EEFDQILDSV LVGPVPAGRH MFVFQADAPN PSLIPETDAV GVTVVLITCT YHGQEFIRVG YYVNNEYLNP ELRENPPMKP DFSQLQRNIL ASNPRVTRFH INWDNNMDRL EAIETQDPSL GCGLPLNCTPIKGLGLPGCI PGLLPENSMD CILEHHHHHH

ASF1 Anti-Silencing Function 1 Homolog B (ASF1B) belongs to the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 protein in yeast. ASF1B is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may have a crucial role in modulating the nucleosome structure of chromatin by guaranteeing a regular supply of histones at sites of nucleosome assembly. ASF1B cooperates with CAF-1 (chromatin assembly factor 1) to stimulate replication-dependent chromatin assembly. ASF1B is highly expressed in the testis and at lower levels in colon, small intestine and thymus. ASF1B is necessary for spermatogenesis.

ASF1B Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 210 amino acids (1-202 a.a) and having a molecular mass of 23.4kDa.ASF1B is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:ASF1B: Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA. Required for spermatogenesis. Interacts with histone H3 (including both histone H3.1 and H3.3) and histone H4. Interacts with the CHAF1A, CHAF1B and RBBP4 subunits of the CAF-1 complex. Interacts with HAT1, NASP, TAF1, TLK1 and TLK2. Highly expressed in testis and at lower levels in colon, small intestine and thymus. Belongs to the ASF1 family.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 19p13.12

Cellular Component: nuclear chromatin; nucleoplasm; protein complex

Molecular Function:histone binding; protein binding

Biological Process: cell differentiation; chromatin modification; DNA replication-dependent nucleosome assembly; DNA replication-independent nucleosome assembly; multicellular organismal development; regulation of transcription, DNA-dependent; spermatogenesis; transcription, DNA-dependent

NCBI Summary:This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. [provided by RefSeq, Jul 2008]
UniProt Code:Q9NVP2
NCBI GenInfo Identifier:74734533
NCBI Gene ID:55723
NCBI Accession:Q9NVP2.1
UniProt Secondary Accession:Q9NVP2,Q53G51, Q9NVZ0,
UniProt Related Accession:Q9NVP2
Molecular Weight:22,434 Da
NCBI Full Name:Histone chaperone ASF1B
NCBI Synonym Full Names:anti-silencing function 1B histone chaperone
NCBI Official Symbol:ASF1B��
NCBI Official Synonym Symbols:CIA-II��
NCBI Protein Information:histone chaperone ASF1B
UniProt Protein Name:Histone chaperone ASF1B
UniProt Synonym Protein Names:Anti-silencing function protein 1 homolog B; hAsf1; hAsf1b; CCG1-interacting factor A-II; CIA-II; hCIA-II
Protein Family:Histone chaperone
UniProt Gene Name:ASF1B��
UniProt Entry Name:ASF1B_HUMAN
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