Human ASF1A Recombinant Protein (RPPB2805)

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SKU:
RPPB2805
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9Y294

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human ASF1A Recombinant Protein
Product Code:RPPB2805
Size:20µg
Species:Human
Target:ASF1A
Synonyms:CGI-98, HSPC146, DKFZp547E2110, ASF1A, Histone chaperone ASF1A, Anti-silencing function protein 1 homolog A, hAsf1, hAsf1a, CCG1-interacting factor A, CIA, hCIA.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:The ASF1A protein solution contains 20mM Tris-HCl, pH-8, 1mM DTT and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAKV QVNNVVVLDN PSPFYNPFQF EITFECIEDL SEDLEWKIIY VGSAESEEYD QVLDSVLVGP VPAGRHMFVF QADAPNPGLI PDADAVGVTV VLITCTYRGQ EFIRVGYYVN NEYTETELRE NPPVKPDFSK LQRNILASNPRVTRFHINWE DNTEKLEDAE SSNPNLQSLL STDALPSASK GWSTSENSLN VMLESHMDCM

ASF1A is part of the H3/H4 family of histone chaperone proteins corresponding to the anti-silencing function-1 gene in yeast. ASF1A is an important element of the histone donor complex that functions in nucleosome assembly. ASF1A interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. Deletion of ASF1A in yeast and Drosophila confers sensitivity to various DNA damaging agents and inhibitors of DNA replication, increases genomic instability and sister chromatid exchange, and activates the DNA damage checkpoint.

ASF1A Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 240 amino acids (1-204 a.a.) and having a molecular mass of 27kDa.ASF1A is fused to a 36 amino acid His Tag and purified by proprietary chromatographic techniques.

UniProt Protein Function:ASF1A: Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit. Interacts with histone H3 (including both histone H3.1 and H3.3) and histone H4. Interacts with the CHAF1A, CHAF1B and RBBP4 subunits of the CAF-1 complex. Interacts with CABIN1, HAT1, HIRA, NASP, TAF1, TLK1, TLK2 and UBN1. Ubiquitously expressed. Belongs to the ASF1 family.
UniProt Protein Details:

Protein type:DNA repair, damage; Histone-binding

Chromosomal Location of Human Ortholog: 6q22.31

Cellular Component: nuclear chromatin; nucleoplasm; nucleus; protein complex

Molecular Function:chromatin binding; histone binding; protein binding

Biological Process: chromatin modification; DNA repair; DNA replication-dependent nucleosome assembly; DNA replication-independent nucleosome assembly; muscle cell differentiation; negative regulation of chromatin silencing; nucleosome assembly; osteoblast differentiation; transcription, DNA-dependent

NCBI Summary:This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. [provided by RefSeq, Jul 2008]
UniProt Code:Q9Y294
NCBI GenInfo Identifier:74735206
NCBI Gene ID:25842
NCBI Accession:Q9Y294.1
UniProt Secondary Accession:Q9Y294,Q6IA08, Q9P014,
UniProt Related Accession:Q9Y294
Molecular Weight:22,969 Da
NCBI Full Name:Histone chaperone ASF1A
NCBI Synonym Full Names:anti-silencing function 1A histone chaperone
NCBI Official Symbol:ASF1A��
NCBI Official Synonym Symbols:CIA; CGI-98; HSPC146��
NCBI Protein Information:histone chaperone ASF1A
UniProt Protein Name:Histone chaperone ASF1A
UniProt Synonym Protein Names:Anti-silencing function protein 1 homolog A; hAsf1; hAsf1a; CCG1-interacting factor A; CIA; hCIA
Protein Family:Histone chaperone
UniProt Gene Name:ASF1A��
UniProt Entry Name:ASF1A_HUMAN
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Epigenetics & Nuclear Signaling Recombinant Proteins

system_update_altDatasheetHuman ASF1A Recombinant Protein Human Histone Chaperone ASF1A (ASF1A) belongs to the H3/H4 family of histone chaperone proteins. ASF1A is ubiquitously expressed in many