Enzymes Recombinant Proteins

Human ARSG Recombinant Protein (RPPB1414)

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RPPB1414

Description

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Product Name:Human ARSG Recombinant Protein
Product Code:RPPB1414
Size:20µg
Species:Human
Target:ARSG
Synonyms:Arylsulfatase G, ASG, ARSG, KIAA1001, UNQ839/PRO1777.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:The ARSG solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSGFLYPLV DFCISGKTRG QKPNFVIILA DDMGWGDLGA NWAETKDTAN LDKMASEGMR FVDFHAAAST CSPSRASLLT GRLGLRNGVT RNFAVTSVGG LPLNETTLAE VLQQAGYVTG IIGKWHLGHH GSYHPNFRGF DYYFGIPYSH DMGCTDTPGY NHPPCPACPQ GDGPSRNLQR DCYTDVALPL YENLNIVEQP VNLSSLAQKY AEKATQFIQR ASTSGRPFLL YVALAHMHVP LPVTQLPAAP RGRSLYGAGL WEMDSLVGQI KDKVDHTVKE NTFLWFTGDN GPWAQKCELA GSVGPFTGFW QTRQGGSPAK QTTWEGGHRV PALAYWPGRV PVNVTSTALL SVLDIFPTVV ALAQASLPQG RRFDGVDVSE VLFGRSQPGH RVLFHPNSGA AGEFGALQTV RLERYKAFYI TGGARACDGS TGPELQHKFP LIFNLEDDTA EAVPLERGGA EYQAVLPEVR KVLADVLQDI ANDNISSADY TQDPSVTPCC NPYQIACRCQ AA

Arylsulfatase G (ARSG) is a member of the sulfatase enzyme family. Sulfatases are enzymes which hydrolyze sulfate esters from sulfated steroids, carbohydrates, proteoglycans, and glycolipids. Sulfatases are involved in hormone biosynthesis, modulation of cell signaling, and degradation of macromolecules. The ARSG protein exhibits arylsulfatase activity at acidic pH, as is characteristic of lysosomal sulfatases. The Arylsulfatase G enzyme localizes in the lysosomes. ARSG is inhibited by phosphate, which forms a covalent bond with the active site 3-oxoalanine. Arylsulfatase G is widely expressed, having very low expression in the brain, lung, heart and skeletal muscle.

ARSG Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 532 amino acids (17-525) and having a molecular mass of 57kDa.ARSG is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:ARSG: Displays arylsulfatase activity at acidic pH with pseudosubstrates, such as p-nitrocatechol sulfate and also, but with lower activity, p-nitrophenyl sulfate and 4- methylumbelliferyl sulfate. Belongs to the sulfatase family.
UniProt Protein Details:

Protein type:Kinase, other; EC 3.1.6.-; Hydrolase; Secreted; Secreted, signal peptide; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 17q24.2

Cellular Component: endoplasmic reticulum; endoplasmic reticulum lumen; extracellular space; lysosome

Molecular Function:arylsulfatase activity; metal ion binding

Biological Process: cellular protein metabolic process; glycosphingolipid metabolic process; post-translational protein modification; sphingolipid metabolic process; sulfur metabolic process

NCBI Summary:The protein encoded by this gene belongs to the sulfatase enzyme family. Sulfatases hydrolyze sulfate esters from sulfated steroids, carbohydrates, proteoglycans, and glycolipids. They are involved in hormone biosynthesis, modulation of cell signaling, and degradation of macromolecules. This protein displays arylsulfatase activity at acidic pH, as is typical of lysosomal sulfatases, and has been shown to localize in the lysosomes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2012]
UniProt Code:Q96EG1
NCBI GenInfo Identifier:74731559
NCBI Gene ID:22901
NCBI Accession:Q96EG1.1
UniProt Secondary Accession:Q96EG1,Q6UXF2, Q9Y2K4,
UniProt Related Accession:Q96EG1
Molecular Weight:57,061 Da
NCBI Full Name:Arylsulfatase G
NCBI Synonym Full Names:arylsulfatase G
NCBI Official Symbol:ARSG��
NCBI Protein Information:arylsulfatase G
UniProt Protein Name:Arylsulfatase G
Protein Family:Arylsulfatase
UniProt Gene Name:ARSG��
UniProt Entry Name:ARSG_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q96EG1
Research Area:
Enzymes
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