Human ARL3 Recombinant Protein (RPPB2789)
- SKU:
- RPPB2789
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P36405
Description
| Product Name: | Human ARL3 Recombinant Protein |
| Product Code: | RPPB2789 |
| Size: | 20µg |
| Species: | Human |
| Target: | ARL3 |
| Synonyms: | ADP-ribosylation factor-like protein 3, ARL3, ARFL3. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | ARL3 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 30% glycerol, 0.1M NaCl, 1mM DTT and 0.1mM PMSF. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMGLLSI LRKLKSAPDQ EVRILLLGLD NAGKTTLLKQ LASEDISHIT PTQGFNIKSV QSQGFKLNVW DIGGQRKIRP YWKNYFENTD ILIYVIDSAD RKRFEETGQE LAELLEEEKL SCVPVLIFAN KQDLLTAAPA SEIAEGLNLH TIRDRVWQIQ SCSALTGEGV QDGMNWVCKN VNAKKK |
ARL3 (ARFL3) belongs to the ARF-like protein (ARL) subfamily of small GTPases. ARL3 does not activate the cholera toxin ADP-ribosyltranferase contrasting from ARFs. ARL3 is ubiquitously expressed. ARL3 may have a role in maintaining rod photoreceptor cells and regulating microtubule-dependent processes. ARL3 knockdown leads to altered cell morphology, failure of cytokinesis, acetylation of alpha tubulin and an increase in binucleated cells.
ARL3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 206 amino acids (1-182 a.a.) and having a molecular mass of 23kDa.ARL3 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | ARL3: Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). Required for normal cytokinesis and cilia signaling. Requires assistance from GTPase- activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms. Required for targeting proteins such as NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium. Does not act as an allosteric activator of the cholera toxin catalytic subunit. Belongs to the small GTPase superfamily. Arf family. |
| UniProt Protein Details: | Protein type:Cytoskeletal; Cell cycle regulation; G protein, monomeric; G protein, monomeric, ARF Chromosomal Location of Human Ortholog: 10q23.3 Cellular Component: Golgi membrane; Golgi apparatus; centrosome; cytoplasmic microtubule; spindle microtubule; midbody; nucleus; photoreceptor connecting cilium Molecular Function:GTPase activity; protein binding; GDP binding; GTP binding; microtubule binding; metal ion binding Biological Process: photoreceptor cell development; protein transport; smoothened signaling pathway; metabolic process; small GTPase mediated signal transduction; organelle organization and biogenesis; intraflagellar transport; cytokinesis; post-Golgi vesicle-mediated transport; kidney development |
| NCBI Summary: | ADP-ribosylation factor-like 3 is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL3 binds guanine nucleotides but lacks ADP-ribosylation factor activity. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P36405 |
| NCBI GenInfo Identifier: | 543851 |
| NCBI Gene ID: | 403 |
| NCBI Accession: | P36405.2 |
| UniProt Secondary Accession: | P36405,Q53X83, Q5JSM2, B2R6C7, |
| UniProt Related Accession: | P36405 |
| Molecular Weight: | 182 |
| NCBI Full Name: | ADP-ribosylation factor-like protein 3 |
| NCBI Synonym Full Names: | ADP-ribosylation factor-like 3 |
| NCBI Official Symbol: | ARL3�� |
| NCBI Official Synonym Symbols: | ARFL3�� |
| NCBI Protein Information: | ADP-ribosylation factor-like protein 3; ARF-like 3 |
| UniProt Protein Name: | ADP-ribosylation factor-like protein 3 |
| Protein Family: | ADP-ribosylation factor-like protein |
| UniProt Gene Name: | ARL3�� |
| UniProt Entry Name: | ARL3_HUMAN |
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