Human ARFIP2 Recombinant Protein (RPPB2780)

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SKU:
RPPB2780
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P53365

Description

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Product Name:Human ARFIP2 Recombinant Protein
Product Code:RPPB2780
Size:20µg
Species:Human
Target:ARFIP2
Synonyms:ADP-ribosylation factor interacting protein 2, partner of RAC1 (arfaptin 2), Partner of RAC1, Protein POR1, arfaptin-2, POR1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The ARFIP2 solution (0.25mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 1mM DTT and 40% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGS TDGILG KAATMEIPIH GNGEARQLPE DDGLEQDLQQ VMVSGPNLNE TSIVSGGYGG SGDGLIPTGS GRHPSHSTTP SGPGDEVARG IAGEKFDIVK KWGINTYKCT KQLLSERFGR GSRTVDLELE LQIELLRETK RKYESVLQLG RALTAHLYSL LQTQHALGDA FADLSQKSPE LQEEFGYNAE TQKLLCKNGE TLLGAVNFFV SSINTLVTKT MEDTLMTVKQ YEAARLEYDA YRTDLEELSL GPRDAGTRGR LESAQATFQA HRDKYEKLRG DVAIKLKFLE ENKIKVMHKQ LLLFHNAVSA YFAGNQKQLE QTLQQFNIKL RPPGAEKPSW LEEQ

Arfaptin 2 (ARFIP2) is a Rac1 binding protein essential for Rac-mediated actin polymerization and the succeeding formation of membrane ruffles and lamellipodia. ARFIP2 is a putative target protein of ADP-ribosylation factor. ARFIP2 is also involved in membrane ruffling. ARFIP2 expression is increased at sites of neurodegeneration. In addition, ARFIP2 interacts with the ADP ribosylation factor ARF6, a GTPase which associates with the plasma membrane and intracellular endosome vesicles, in a GTP dependent mode. Furthermore, Arfaptin 2 controls the aggregation of mutant Huntingtin protein by weakening proteasome function.

ARFIP2 Human Recombinant produced in E. coli is a single polypeptide chain containing 364 amino acids (1-341) and having a molecular mass of 40.2 kDa.ARFIP2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:arfaptin 2: Putative target protein of ADP-ribosylation factor. Involved in membrane ruffling. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:GAPs, Rac/Rho; Motility/polarity/chemotaxis; GAPs

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: ruffle; cytoplasm; plasma membrane; trans-Golgi network membrane; cell cortex

Molecular Function:protein domain specific binding; protein binding; GTP binding; GTP-dependent protein binding; Rac GTPase binding

Biological Process: lamellipodium biogenesis; small GTPase mediated signal transduction; ruffle organization and biogenesis; actin cytoskeleton organization and biogenesis; cell motility

UniProt Code:P53365
NCBI GenInfo Identifier:1703205
NCBI Gene ID:23647
NCBI Accession:P53365.1
UniProt Secondary Accession:P53365,B4DX86, B4E306, D3DQT5,
UniProt Related Accession:P53365
Molecular Weight:33,782 Da
NCBI Full Name:Arfaptin-2
NCBI Synonym Full Names:ADP-ribosylation factor interacting protein 2
NCBI Official Symbol:ARFIP2��
NCBI Official Synonym Symbols:POR1��
NCBI Protein Information:arfaptin-2; partner of RAC1 (arfaptin 2)
UniProt Protein Name:Arfaptin-2
UniProt Synonym Protein Names:ADP-ribosylation factor-interacting protein 2; Partner of RAC1; Protein POR1
Protein Family:Arfaptin
UniProt Gene Name:ARFIP2��
UniProt Entry Name:ARFP2_HUMAN
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