Description
| Product Name: | Human ARF5 Recombinant Protein |
| Product Code: | RPPB2776 |
| Size: | 20µg |
| Species: | Human |
| Target: | ARF5 |
| Synonyms: | ADP-ribosylation factor 5, ARF5. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | ARF5 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 20% glycerol and 0.1M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGLTVSALFS RIFGKKQMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN ICFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV QESADELQKM LQEDELRDAV LLVFANKQDM PNAMPVSELT DKLGLQHLRS RTWYVQATCA TQGTGLYDGL DWLSHELSKR |
ADP-ribosylation factor 5 (ARF5) is a small guanine nucleotide-binding protein which enhances the enzymatic activities of cholera toxin. ARF-dependent regulatory mechanisms include the coordination of spectrin interactions with golgi membranes and the connection of actin to the golgi via rho family-dependent G-protein localization and WASP/Arp2/3 complexes. ARF5 is involved in vesicular transport and functioning via phospholipase D activation.
ARF5 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 200 amino acids (1-180 a.a) and having a molecular mass of 22.6kDa.ARF5 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | ARF5: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Belongs to the small GTPase superfamily. Arf family. |
| UniProt Protein Details: | Protein type:G protein, monomeric, ARF; G protein; G protein, monomeric; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 7q31.3 Cellular Component: Golgi apparatus; perinuclear region of cytoplasm; plasma membrane Molecular Function:GTPase activity; protein binding; GTP binding Biological Process: vesicle-mediated transport; protein transport; metabolic process; small GTPase mediated signal transduction |
| NCBI Summary: | This gene is a member of the human ADP-ribosylation factor (ARF) gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. [provided by RefSeq, Dec 2010] |
| UniProt Code: | P84085 |
| NCBI GenInfo Identifier: | 51316990 |
| NCBI Gene ID: | 381 |
| NCBI Accession: | P84085.2 |
| UniProt Secondary Accession: | P84085,P26437, |
| UniProt Related Accession: | P84085 |
| Molecular Weight: | 180 |
| NCBI Full Name: | ADP-ribosylation factor 5 |
| NCBI Synonym Full Names: | ADP-ribosylation factor 5 |
| NCBI Official Symbol: | ARF5�� |
| NCBI Protein Information: | ADP-ribosylation factor 5 |
| UniProt Protein Name: | ADP-ribosylation factor 5 |
| Protein Family: | Auxin response factor |
| UniProt Gene Name: | ARF5�� |
| UniProt Entry Name: | ARF5_HUMAN |
