Human ARF4 Recombinant Protein (RPPB2775)
- SKU:
- RPPB2775
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P18085
Description
| Product Name: | Human ARF4 Recombinant Protein |
| Product Code: | RPPB2775 |
| Size: | 20µg |
| Species: | Human |
| Target: | ARF4 |
| Synonyms: | ADP-ribosylation factor 4, ARF4, ARF2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The ARF4 solution (0.5 mg/ml) in 20mM Tris-HCl buffer (pH8.0), 0.2M NaCl, 5mM DTT and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMGLTIS SLFSRLFGKK QMRILMVGLD AAGKTTILYK LKLGEIVTTI PTIGFNVETV EYKNICFTVW DVGGQDRIRP LWKHYFQNTQ GLIFVVDSND RERIQEVADE LQKMLLVDEL RDAVLLLFAN KQDLPNAMAI SEMTDKLGLQ SLRNRTWYVQ ATCATQGTGL YEGLDWLSNE LSKR |
ARF4 belongs to the ARF gene family whose members encode small guanine nucleotide-binding proteins which stimulate the ADP-ribosyltransferase activity of cholera toxin and have a role in vesicular trafficking and as activators of phospholipase D. The ARF proteins include five ARF proteins and eleven ARF-like proteins and constitute one family of the RAS superfamily. They are classified as class I, class II and class III; the ARF4 gene is a class II member. The members of each class share a common gene organization. The ARF4 gene spans approximately 12kb and contains 6 exons and 5 introns. The ARF4 gene is the most divergent member of the human ARFs.
ARF4 Human Recombinant fused with a 24 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 204 amino acids (1-180 a.a.) and having a molecular mass of 23kDa. The ARF4 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | ARF4: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Belongs to the small GTPase superfamily. Arf family. |
| UniProt Protein Details: | Protein type:G protein, monomeric; Motility/polarity/chemotaxis; G protein, monomeric, ARF; G protein Chromosomal Location of Human Ortholog: 3p14.3 Cellular Component: cytosol; dendritic spine; membrane Molecular Function:ARF guanyl-nucleotide exchange factor activity; epidermal growth factor receptor binding; GTP binding; GTPase activity; protein binding Biological Process: cell migration; ER to Golgi vesicle-mediated transport; negative regulation of apoptosis; phospholipase D activation; positive regulation of transcription from RNA polymerase II promoter; protein amino acid ADP-ribosylation; retrograde vesicle-mediated transport, Golgi to ER |
| NCBI Summary: | This gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. The ARF proteins are categorized as class I, class II and class III; this gene is a class II member. The members of each class share a common gene organization. The ARF4 gene spans approximately 12kb and contains six exons and five introns. This gene is the most divergent member of the human ARFs. Conflicting map positions at 3p14 or 3p21 have been reported for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P18085 |
| NCBI GenInfo Identifier: | 114123 |
| NCBI Gene ID: | 378 |
| NCBI Accession: | P18085.3 |
| UniProt Secondary Accession: | P18085,P21371, B2R7J7, |
| UniProt Related Accession: | P18085 |
| Molecular Weight: | 20511 Da |
| NCBI Full Name: | ADP-ribosylation factor 4 |
| NCBI Synonym Full Names: | ADP ribosylation factor 4 |
| NCBI Official Symbol: | ARF4�� |
| NCBI Official Synonym Symbols: | ARF2�� |
| NCBI Protein Information: | ADP-ribosylation factor 4 |
| UniProt Protein Name: | ADP-ribosylation factor 4 |
| Protein Family: | Auxin response factor |
| UniProt Gene Name: | ARF4�� |
| UniProt Entry Name: | ARF4_HUMAN |
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