Human ARF3 Recombinant Protein (RPPB2774)

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SKU:
RPPB2774
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P61204

Description

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Product Name:Human ARF3 Recombinant Protein
Product Code:RPPB2774
Size:20µg
Species:Human
Target:ARF3
Synonyms:ADP-ribosylation factor 3, ARF3.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:ARF3 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGNIFGNLLK SLIGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV NEAREELMRM LAEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRH RNWYIQATCA TSGDGLYEGL DWLANQLKNK K

ADP-ribosylation factor 3 (ARF3) belongs to the human ARF gene family. This family encodes small guanine nucleotide-binding proteins which stimulate the ADP-ribosyltransferase activity of cholera toxin and have a role in vesicular trafficking and as activators of phospholipase D. ARF3 functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. ARF3 is involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. The ARF3 gene is comprised of 5 exons and 4 introns.

ARF3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 201 amino acids (1-181 a.a.) and having a molecular mass of 22.8kDa.ARF3 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:ARF3: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Belongs to the small GTPase superfamily. Arf family.
UniProt Protein Details:

Protein type:G protein, monomeric, ARF; G protein; G protein, monomeric; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: Golgi membrane

Molecular Function:GTPase activity

Biological Process: phosphatidylinositol biosynthetic process; retrograde vesicle-mediated transport, Golgi to ER

NCBI Summary:ADP-ribosylation factor 3 (ARF3) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6) and members of each class share a common gene organization. The ARF3 gene contains five exons and four introns. [provided by RefSeq, Jul 2008]
UniProt Code:P61204
NCBI GenInfo Identifier:47117657
NCBI Gene ID:377
NCBI Accession:P61204.2
UniProt Secondary Accession:P61204,P16587, A8K6G8, B7ZB63,
UniProt Related Accession:P61204
Molecular Weight:16,145 Da
NCBI Full Name:ADP-ribosylation factor 3
NCBI Synonym Full Names:ADP ribosylation factor 3
NCBI Official Symbol:ARF3��
NCBI Protein Information:ADP-ribosylation factor 3
UniProt Protein Name:ADP-ribosylation factor 3
Protein Family:Auxin response factor
UniProt Gene Name:ARF3��
UniProt Entry Name:ARF3_HUMAN
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