Human Apoptosis Signaling Array (19 targets) (SARB0022)

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SKU:
SARB0022

Description

system_update_altDatasheet
Product Sku:SARB0022
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:19
Gene Symbols:AKT1, ATM, BAD, CASP3, CASP7, CHEK1, CHEK2, EIF2A, HSPB1, MAP3K7|MAPK1, MAPK3, MAPK8, NFKBIA, PARP1, PSMD9, RELA, SMAD2, TP53
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:Akt Signaling / HIF-1 alpha Signaling / IGF Signaling / JAK/STAT Signaling / MAPK Signaling / mTOR Signaling / NFkB Signaling / p53 Signaling / PI3K-AKT Signaling / TGF-beta Signaling / Wnt/beta-Catenin Signaling
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
Akt (P-Ser473) (PKB (P-Ser473))
  • Gene Symbol: AKT1 PKB RAC
  • Gene ID: 207
  • Accession #: P31749
ATM (P-Ser1981)
  • Gene Symbol: ATM
  • Gene ID: 472
  • Accession #: Q13315
BAD (P-Ser112)
  • Gene Symbol: BAD BBC6 BCL2L8
  • Gene ID: 572
  • Accession #: Q92934
Caspase-3 (Cleaved Asp175)
  • Gene Symbol: CASP3 CPP32
  • Gene ID: 836
  • Accession #: P42574
Caspase-7 (Cleaved Asp198)
  • Gene Symbol: CASP7 MCH3
  • Gene ID: 840
  • Accession #: P55210
CHK1 (P-Ser296)
  • Gene Symbol: CHEK1 CHK1
  • Gene ID: 1111
  • Accession #: O14757
CHK2 (P-Thr68)
  • Gene Symbol: CHEK2 CDS1 CHK2 RAD53
  • Gene ID: 11200
  • Accession #: O96017
eIF-2a (P-Ser52)
  • Gene Symbol: EIF2A CDA02 MSTP004 MSTP089
  • Gene ID: 83939
  • Accession #: Q9BY44
ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187)
  • Gene Symbol: MAPK3 ERK1 PRKM3/MAPK1 ERK2 PRKM1 PRKM2
  • Gene ID: 5595/5594
  • Accession #: P27361/P28482
HSP27 (P-Ser82)
  • Gene Symbol: HSPB1 HSP27 HSP28
  • Gene ID: 3315
  • Accession #: P04792
IKBa (P-Ser32)
  • Gene Symbol: NFKBIA IKBA MAD3 NFKBI
  • Gene ID: 4792
  • Accession #: P25963
JNK (P-Thr183/Tyr185)
  • Gene Symbol: MAPK8 JNK1 PRKM8 SAPK1 SAPK1C
  • Gene ID: 5599
  • Accession #: P45983
NFKBP65 (P-Ser536)
  • Gene Symbol: RELA NFKB3
  • Gene ID: 5970
  • Accession #: Q04206
PARP1 (Cleaved Asp214/Gly215)
  • Gene Symbol: PARP1 ADPRT PPOL
  • Gene ID: 142
  • Accession #: P09874
p27 (P-Thr198)
  • Gene Symbol: PSMD9
  • Gene ID: 5715
  • Accession #: O00233
P53 (P-Ser15)
  • Gene Symbol: TP53 P53
  • Gene ID: 6198
  • Accession #: P23443
SMAD2 (Ser245/250/255)
  • Gene Symbol: SMAD2 MADH2 MADR2
  • Gene ID: 4087
  • Accession #: Q15796
TAK1 (P-Ser412)
  • Gene Symbol: MAP3K7 TAK1
  • Gene ID: 6885
  • Accession #: O43318
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human Apoptosis Signaling Antibody Array C1 Membranes
  • Blocking Buffer
  • Detection Antibody Cocktail
  • 1,000X HRP-Anti-Rabbit IgG Concentrate
  • 20X Wash Buffer I Concentrate
  • 20X Wash Buffer II Concentrate
  • 2X Cell Lysis Buffer Concentrate
  • Detection Buffer C
  • Detection Buffer D
  • 8-Well Incubation Tray w/ Lid
  • Protease Inhibitor Cocktail
  • 100x Phosphatase Inhibitor Cocktail I
  • Phosphatase Inhibitor Cocktail II
  • Plastic Sheets
  • Array Map Template

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Detection Antibody Cocktail
4.Incubate with HRP-Conjugated anti-IgG
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product Type:
Protein Array
Analytes:
Akt (P-Ser473) (PKB (P-Ser473))
Analytes:
ATM (P-Ser1981)
Analytes:
BAD (P-Ser112)
Analytes:
Caspase-3 (Cleaved Asp175)
Analytes:
Caspase-7 (Cleaved Asp198)
Analytes:
CHK1 (P-Ser296)
Analytes:
CHK2 (P-Thr68)
Analytes:
eIF-2a (P-Ser52)
Analytes:
ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187)
Analytes:
HSP27 (P-Ser82)
Analytes:
IKBa (P-Ser32)
Analytes:
JNK (P-Thr183/Tyr185)
Analytes:
NFKBP65 (P-Ser536)
Analytes:
PARP1 (Cleaved Asp214/Gly215)
Analytes:
p27 (P-Thr198)
Analytes:
P53 (P-Ser15)
Analytes:
SMAD2 (Ser245/250/255)
Analytes:
TAK1 (P-Ser412)
Reactivity:
Human
Applications:
Multiplex Array
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