Human Apoptosis Array (43 targets) (SARB0021)

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SKU:
SARB0021
Product Type:
Protein Array
Analytes:
bad
Analytes:
bax
Analytes:
bcl-2
Analytes:
bcl-w
Analytes:
BID
Analytes:
BIM
Analytes:
Caspase-3
Analytes:
Caspase-8
Analytes:
CD40 (TNFRSF5)
Analytes:
CD40 Ligand (TNFSF5)
Analytes:
cIAP-2
Analytes:
Cytochrome C
Analytes:
DR6 (TNFRSF21)
Analytes:
Fas (TNFRSF6/Apo-1)
Analytes:
Fas Ligand (TNFSF6)
Analytes:
HSP27
Analytes:
HSP60
Analytes:
HSP70
Analytes:
HTRA2
Analytes:
IGF-1
Analytes:
IGF-2
Analytes:
IGFBP-1
Analytes:
IGFBP-2
Analytes:
IGFBP-3
Analytes:
IGFBP-4
Analytes:
IGFBP-5
Analytes:
IGFBP-6
Analytes:
IGF-1 R
Analytes:
livin
Analytes:
p21
Analytes:
p27
Analytes:
p53
Analytes:
SMAC
Analytes:
Survivin (BIRC5)
Analytes:
TNF RI (TNFRSF1A)
Analytes:
TNF RII (TNFRSF1B)
Analytes:
TNF alpha
Analytes:
TNF beta (TNFSF1B)
Analytes:
TRAIL R1 (TNFRSF10A/DR4)
Analytes:
TRAIL R2 (TNFRSF10B/DR5)
Analytes:
TRAIL R3 (TNFRSF10C)
Analytes:
TRAIL R4 (TNFRSF10D)
Analytes:
XIAP
Reactivity:
Human
Applications:
Multiplex Array

Description

system_update_altDatasheet
Product Sku:SARB0021
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:43
Gene Symbols:BAD, BAX, BCL2, BCL2L11, BCL2L2, BID, BIRC3, BIRC5, BIRC7, CASP3|CASP8, CD40, CD40LG, CDKN1A, CYCS, DIABLO, FAS, FASLG, HSPA14, HSPA4, HSPB1, HTRA2, IGF1, IGF1R, IGF2, IGFBP1, IGFBP2, IGFBP3, IGFBP4, IGFBP5, IGFBP6, LTA, SSSCA1, TNF, TNFRSF10A, TNFRSF10B, TNFRSF10C, TNFRSF10D, TNFRSF1A, TNFRSF1B, TNFRSF21, TP53, XIAP
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:Akt Signaling / IGF Signaling / p53 Signaling
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
bad
  • Gene Symbol: bad
  • Gene ID: 572
  • Accession #: Q92934
bax
  • Gene Symbol: BAX
  • Gene ID: 581
  • Accession #: Q07812
bcl-2
  • Gene Symbol: BCL2
  • Gene ID: 596
  • Accession #: P10415
bcl-w
  • Gene Symbol: BCL2L2
  • Gene ID: 599
  • Accession #: Q92843
BID
  • Gene Symbol: BID
  • Gene ID: 637
  • Accession #: P55957
BIM
  • Gene Symbol: BCL2L11
  • Gene ID: 10018
  • Accession #: Q43521
Caspase-3
  • Gene Symbol: CASP3
  • Gene ID: 836
  • Accession #: P42574
Caspase-8
  • Gene Symbol: CASP8
  • Gene ID: 841
  • Accession #: Q14790
CD40 (TNFRSF5)
  • Gene Symbol: CD40
  • Gene ID: 958
  • Accession #: P25942
CD40 Ligand (TNFSF5)
  • Gene Symbol: CD40LG
  • Gene ID: 959
  • Accession #: P29965
cIAP-2
  • Gene Symbol: BIRC3
  • Gene ID: 330
  • Accession #: Q13489
Cytochrome C
  • Gene Symbol: CYCS
  • Gene ID: 54205
  • Accession #: P99999
DR6 (TNFRSF21)
  • Gene Symbol: TNFRSF21
  • Gene ID: 27242
  • Accession #: O75509
Fas (TNFRSF6/Apo-1)
  • Gene Symbol: Fas
  • Gene ID: 355
  • Accession #: P25445
Fas Ligand (TNFSF6)
  • Gene Symbol: FASLG
  • Gene ID: 356
  • Accession #: P48023
HSP27
  • Gene Symbol: HSPB1
  • Gene ID: 3315
  • Accession #: P04792
HSP60
  • Gene Symbol: HSPA14
  • Gene ID: 51182
  • Accession #: Q0VDF9
HSP70
  • Gene Symbol: HSPA4
  • Gene ID: 3308
  • Accession #: P34932
HTRA2
  • Gene Symbol: HTRA2
  • Gene ID: 27429
  • Accession #: O43464
IGF-1
  • Gene Symbol: IGF1
  • Gene ID: 3479
  • Accession #: P05019
IGF-2
  • Gene Symbol: Igf2
  • Gene ID: 3481
  • Accession #: P01344
IGFBP-1
  • Gene Symbol: IGFBP1
  • Gene ID: 3484
  • Accession #: P08833
IGFBP-2
  • Gene Symbol: IGFBP2
  • Gene ID: 3485
  • Accession #: P18065
IGFBP-3
  • Gene Symbol: IGFBP3
  • Gene ID: 3486
  • Accession #: P17936
IGFBP-4
  • Gene Symbol: IGFBP4
  • Gene ID: 3487
  • Accession #: P22692
IGFBP-5
  • Gene Symbol: IGFBP5
  • Gene ID: 3488
  • Accession #: P24593
IGFBP-6
  • Gene Symbol: IGFBP6
  • Gene ID: 3489
  • Accession #: P24592
IGF-1 R
  • Gene Symbol: IGF1R
  • Gene ID: 3480
  • Accession #: P08069
livin
  • Gene Symbol: BIRC7
  • Gene ID: 79444
  • Accession #: Q96CA5
p21
  • Gene Symbol: CDKN1A
  • Gene ID: 1026
  • Accession #: P38936
p27
  • Gene Symbol: SSSCA1
  • Gene ID: 10534
  • Accession #: O60232
p53
  • Gene Symbol: TP53
  • Gene ID: 7157
  • Accession #: P04637
SMAC
  • Gene Symbol: DIABLO
  • Gene ID: 56616
  • Accession #: Q9NR28
Survivin (BIRC5)
  • Gene Symbol: BIRC5
  • Gene ID: 332
  • Accession #: O15392
TNF RI (TNFRSF1A)
  • Gene Symbol: TNFRSF1A
  • Gene ID: 7132
  • Accession #: P19438
TNF RII (TNFRSF1B)
  • Gene Symbol: TNFRSF1B
  • Gene ID: 7133
  • Accession #: P20333
TNF alpha
  • Gene Symbol: TNF
  • Gene ID: 7124
  • Accession #: P01375
TNF beta (TNFSF1B)
  • Gene Symbol: LTA
  • Gene ID: 4049
  • Accession #: P01374
TRAIL R1 (TNFRSF10A/DR4)
  • Gene Symbol: TNFRSF10A
  • Gene ID: 8797
  • Accession #: O00220
TRAIL R2 (TNFRSF10B/DR5)
  • Gene Symbol: TNFRSF10B
  • Gene ID: 8795
  • Accession #: O14763
TRAIL R3 (TNFRSF10C)
  • Gene Symbol: TNFRSF10C
  • Gene ID: 8794
  • Accession #: O14798
TRAIL R4 (TNFRSF10D)
  • Gene Symbol: TNFRSF10D
  • Gene ID: 8793
  • Accession #: Q9UBN6
XIAP
  • Gene Symbol: XIAP
  • Gene ID: 331
  • Accession #: P98170
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human Apoptosis Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • Protease Inhibitor Cocktail
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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