Human Immunology ELISA Kits 4
Human Apolipoprotein C-III (APOC3) ELISA Kit
- SKU:
- HUEB0293
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- P02656
- Range:
- 2.35-150 ng/mL
- ELISA Type:
- Sandwich
- Synonyms:
- ApoC3, Apolipoprotein C3, APOC3, ApoC-III, MGC150353, Apo-CIII, APOCIII, apoC-III
- Reactivity:
- Human
Description
Human Apolipoprotein C-III (APOC3) ELISA Kit
Key Features
| Save Time | Pre-coated 96 well plate | |
| Quick Start | Kit includes all necessary reagents | |
| Publication Ready | Reproducible and reliable results |
Overview
| Product Name: | Human Apolipoprotein C-III (APOC3) ELISA Kit |
| Product Code: | HUEB0293 |
| Size: | 96 Assays |
| Alias: | Apolipoprotein C-III, Apo-CIII, ApoC-III, Apolipoprotein C3, APOC3 |
| Detection Method: | Sandwich |
| Reactivity: | Human |
| Range: | 2.35-150 ng/mL |
| Storage: | 4°C for 6 months |
| Note: | For Research Use Only |
Protein Information
| Uniprot: | P02656 |
| UniProt Protein Function: | APOC3: Inhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles. Defects in APOC3 are the cause of hyperalphalipoproteinemia type 2 (HALP2). HALP2 is a condition characterized by high levels of high density lipoprotein (HDL) and increased HDL cholesterol levels. Belongs to the apolipoprotein C3 family. |
| UniProt Code: | |
| NCBI GenInfo Identifier: | |
| NCBI Gene ID: | |
| NCBI Accession: | |
| UniProt Related Accession: | |
| Molecular Weight: | 10,852 Da |
Kit Components
| Component | Quantity | Storage |
| ELISA Microplate (Dismountable) | 8x12 strips | -20°C |
| Lyophilized Standard | 2 | -20°C |
| Sample Diluent | 20ml | -20°C |
| Assay Diluent A | 10mL | -20°C |
| Assay Diluent B | 10ml | -20°C |
| Detection Reagent A | 60µL | -20°C |
| Detection Reagent B | 120µL | -20°C |
| Wash Buffer (25X) | 30ml | 4°C |
| Substrate | 10mL | 4°C |
| Stop Solution | 10ml | 4°C |
| Plate Sealer | 5 | - |
Other materials required:
- Microplate reader with 450 nm wavelength filter
- Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
- Incubator
- Deionized or distilled water
- Absorbent paper
- Buffer resevoir
Protocol
*Note: Protocols are specific to each batch/lot. For the exact instructions please follow the protocol included in your kit.
Before adding to wells, equilibrate the SABC working solution and TMB substrate for at least 30 min at 37°C. When diluting samples and reagents, they must be mixed completely and evenly. It is recommended to plot a standard curve for each test.
| Step | Procedure |
| 1. | Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells! |
| 2. | Aliquot 0.1ml standard solutions into the standard wells. |
| 3. | Add 0.1 ml of Sample / Standard dilution buffer into the control (zero) well. |
| 4. | Add 0.1 ml of properly diluted sample ( Human serum, plasma, tissue homogenates and other biological fluids.) into test sample wells. |
| 5. | Seal the plate with a cover and incubate at 37 °C for 90 min. |
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