Human APIP Recombinant Protein (RPPB2764)

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SKU:
RPPB2764
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q96GX9

Description

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Product Name:Human APIP Recombinant Protein
Product Code:RPPB2764
Size:20µg
Species:Human
Target:APIP
Synonyms:APAF1 interacting protein, CGI-29, APIP2, Mmrp19, APAF1-interacting protein, MTRu-1-P dehydratase, probable methylthioribulose-1-phosphate dehydratase, CGI29, EC 4.2.1.109, dJ179L10.2, MMRP19.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The APIP solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT and 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMSGCDA REGDCCSRRC GAQDKEHPRY LIPELCKQFY HLGWVTGTGG GISLKHGDEI YIAPSGVQKE RIQPEDMFVC DINEKDISGP SPSKKLKKSQ CTPLFMNAYT MRGAGAVIHT HSKAAVMATL LFPGREFKIT HQEMIKGIKK CTSGGYYRYD DMLVVPIIEN TPEEKDLKDR MAHAMNEYPD SCAVLVRRHG VYVWGETWEK AKTMCECYDY LFDIAVSMKK VGLDPSQLPV GENGIV

APIP catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P). APIP has an anti-apoptotic function and inhibits muscle ischemic impairment. APIP inhibits the cytochrome c-dependent and APAF1-mediated cell death.

APIP Human Recombinant produced E. coli is a single polypeptide chain containing 266 amino acids (1-242) and having a molecular mass of 29.7kDa.APIP is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:MMRP19: Catalyzes the dehydration of methylthioribulose-1- phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1- phosphate (DK-MTP-1-P). Has an anti-apoptotic function and prevents muscle ischemic damage. Inhibits the cytochrome c- dependent and APAF1-mediated cell death. Belongs to the aldolase class II family. MtnB subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 4.2.1.109; Amino Acid Metabolism - cysteine and methionine; Apoptosis

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: cytoplasm

Molecular Function:identical protein binding; 5-methylthioribulose-1-phosphate 4-dehydratase activity; protein binding; zinc ion binding

Biological Process: methionine salvage; sulfur amino acid metabolic process; apoptosis; methionine biosynthetic process from S-adenosylmethionine; polyamine metabolic process; negative regulation of apoptosis

NCBI Summary:APIP is an APAF1 (MIM 602233)-interacting protein that acts as a negative regulator of ischemic/hypoxic injury (Cho et al., 2004 [PubMed 15262985]).[supplied by OMIM, Dec 2008]
UniProt Code:Q96GX9
NCBI GenInfo Identifier:74731866
NCBI Gene ID:51074
NCBI Accession:Q96GX9.1
UniProt Secondary Accession:Q96GX9,Q6PJX6, Q8WVU2, Q96HK2, Q9Y318, A8K9D3,
UniProt Related Accession:Q96GX9
Molecular Weight:242
NCBI Full Name:Methylthioribulose-1-phosphate dehydratase
NCBI Synonym Full Names:APAF1 interacting protein
NCBI Official Symbol:APIP��
NCBI Official Synonym Symbols:APIP2; CGI29; hAPIP; CGI-29; MMRP19��
NCBI Protein Information:methylthioribulose-1-phosphate dehydratase; APIP; MTRu-1-P dehydratase; APAF1-interacting protein; probable methylthioribulose-1-phosphate dehydratase
UniProt Protein Name:Methylthioribulose-1-phosphate dehydratase
UniProt Synonym Protein Names:APAF1-interacting protein
UniProt Gene Name:APIP��
UniProt Entry Name:MTNB_HUMAN
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