Cytokines Recombinant Proteins

Human ANGPTL4 Recombinant Protein (RPPB0041)

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SKU:
RPPB0041
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9BY76
Research Area:
Cytokines

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human ANGPTL4 Recombinant Protein
Product Code:RPPB0041
Size:10µg
Species:Human
Target:ANGPTL4
Synonyms:ANGPTL4, NL2, ARP4, FIAF, PGAR, HFARP, pp1158, ANGPTL2, Fasting- Induced Adipose Factor, Hepatic Fibrinogen/Angiopoietin-Related Protein, PPARG Angiopoietin-Related Protein.
Source:HEK293 Cells
Physical Appearance:Filtered White lyophilized (freeze-dried) powder.
Formulation:Filtered and lyophilized from 0.5mg/ml in 20mM Tris buffer and 50mM NaCl pH-7.5.
Solubility:Add pyrogen free water to a working concentration of 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Stability:Store lyophilized Angiopoietin-like Protein 4 Human recombinant at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:GPVQSKSPRF ASWDEMNVLA HGLLQLGQGL REHAERTRSQ LSALERRLSA CGSACQGTEG STDLPLAPES RVDPEVLHSL QTQLKAQNSR IQQLFHKVAQ QQRHLEKQHL RIQHLQSQFG LLDHKHLDHE VAKPARRKRL PEMAQPVDPA HNVSRLHRLP RDCQELFQVG ERQSGLFEIQ PQGSPPFLVN CKMTSDGGWT VIQRRHDGSV DFNRPWEAYK AGFGDPHGEF WLGLEKVHSI TGDRNSRLAV QLRDWDGNAE LLQFSVHLGG EDTAYSLQLT APVAGQLGAT TVPPSGLSVP FSTWDQDHDL RRDKNCAKSL SGGWWFGTCS HSNLNGQYFR SIPQQRQKLK KGIFWKTWRG RYYPLQATTM LIQPMAAEAA SAAADYKDDDDK

The fasting-induced adipose factor (FIAF, ANGPTL4, PGAR, HFARP) was identified as an adipocytokine up-regulated by fasting, by peroxisome proliferator-activated receptor agonists, and by hypoxia. At the protein level, in human and mouse blood plasma, FIAF was found to be present both as a native protein and in a truncated form. Differentiation of mouse 3T3-L1 adipocytes was associated with the production of truncated FIAF, whereas in human white adipose tissue and SGBS adipocytes, only the native FIAF could be detected. Interestingly, the truncated FIAF was produced by human liver.Experimental data suggest that FIAF is mainly presented in human blood plasma in a truncated form (FIAF-S2), whose level is increased by fenofibrate treatment. Levels of both truncated and native FIAF showed marked inter individual variation but were not associated with body mass index and were not influenced by prolonged semistarvation.

The ANGPTL4 Human Recombinant is manufactured with C-terminal fusion of 11 amino acid FLAG Tag. The ANGPTL4 Flag -Tagged Fusion Protein is a 44.2kDa protein containing 392 amino acid residues of the Angiopoietin-like Protein 4 and 11 additional amino acid residues - Flag Tag (underlined).

UniProt Protein Function:ANGPTL4: Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorigenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation.
UniProt Protein Details:

Protein type:Inhibitor; Apoptosis; Secreted, signal peptide; Secreted; Extracellular matrix

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region

Molecular Function:enzyme inhibitor activity; protein binding

Biological Process: positive regulation of angiogenesis; response to hypoxia; negative regulation of lipoprotein lipase activity; cellular lipid metabolic process; angiogenesis; cell differentiation; protein homooligomerization; negative regulation of apoptosis

Disease: Plasma Triglyceride Level Quantitative Trait Locus

NCBI Summary:This gene encodes a glycosylated, secreted protein containing a C-terminal fibrinogen domain. The encoded protein is induced by peroxisome proliferation activators and functions as a serum hormone that regulates glucose homeostasis, lipid metabolism, and insulin sensitivity. This protein can also act as an apoptosis survival factor for vascular endothelial cells and can prevent metastasis by inhibiting vascular growth and tumor cell invasion. The C-terminal domain may be proteolytically-cleaved from the full-length secreted protein. Decreased expression of this gene has been associated with type 2 diabetes. Alternative splicing results in multiple transcript variants. This gene was previously referred to as ANGPTL2 but has been renamed ANGPTL4. [provided by RefSeq, Sep 2013]
UniProt Code:Q9BY76
NCBI GenInfo Identifier:25008123
NCBI Gene ID:51129
NCBI Accession:Q9BY76.2
UniProt Secondary Accession:Q9BY76,Q53HQ6, Q53HU1, Q6UXN0, Q9HBV4, Q9NZU4, Q9Y5B3 A8MY84, B4E089, D6W670, F5H0I2,
UniProt Related Accession:Q9BY76
Molecular Weight:26,853 Da
NCBI Full Name:Angiopoietin-related protein 4
NCBI Synonym Full Names:angiopoietin-like 4
NCBI Official Symbol:ANGPTL4��
NCBI Official Synonym Symbols:NL2; ARP4; FIAF; HARP; PGAR; HFARP; TGQTL; UNQ171; pp1158; ANGPTL2��
NCBI Protein Information:angiopoietin-related protein 4; fasting-induced adipose factor; PPARG angiopoietin related protein; hepatic angiopoietin-related protein; hepatic fibrinogen/angiopoietin-related protein; peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin-related protein
UniProt Protein Name:Angiopoietin-related protein 4
UniProt Synonym Protein Names:Angiopoietin-like protein 4; Hepatic fibrinogen/angiopoietin-related protein; HFARP
Protein Family:Angiopoietin-related protein
UniProt Gene Name:ANGPTL4��
UniProt Entry Name:ANGL4_HUMAN
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system_update_altDatasheetMouse ANGPTL4 Recombinant Protein Angiopoietin-related protein 4?ANGPTL4?is a secreted protein and contains 1 fibrinogen C-terminal domain. The protein may act as a regu